By Type: Journal Article

Branquinho, R., J. V. Pinto, T. Busani, P. Barquinha, L. Pereira, P. V. Baptista, R. Martins, and E. Fortunato. "Plastic Compatible Sputtered Ta2O5 Sensitive Layer for Oxide Semiconductor TFT Sensors." J. Display Technol 9 (2013): 723-728.
Cordeiro, M., L. Giestas, J. C. Lima, and Pedro Viana Baptista. "Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis." J Biotechnol 168 (2013): 90-94. AbstractWebsite

We previously reported a strategy that combines Förster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.
Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair.

Veigas, Bruno, Pedro Pedrosa, Isabel Couto, Miguel Viveiros, and Pedro V. Baptista. "Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis." Journal of Nanobiotechnology (2013).
Carlos, F. F., J. Silva-Nunes, O. Flores, M. Brito, G. Doria, L. Veiga, and P. V. Baptista. "Association of FTO and PPARG polymorphisms with obesity in Portuguese women." Diabetes Metab Syndr Obes 6 (2013): 241-5. AbstractWebsite

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Fernandes, Alexandra R., and Pedro Viana Baptista. "Nanotechnology for Cancer Diagnostics and Therapy – An Update on Novel Molecular Players." Current Cancer Therapy Reviews 9 (2013): 164-172. AbstractWebsite

Nanotechnology has emerged as a "disruptive technology" that may provide researchers with new and innovative ways to diagnose, treat and monitor cancer. In fact, nanomedicine approaches have delivered several strategies, such as new imaging agents, real-time assessments of therapeutic and surgical efficacy, multifunctional, targeted devices capable of bypassing biological barriers to target and silence specific pathways in tumours. Of particular interest, has been the increased capability to deliver multiple therapeutic agents directly to bulk cancer cells and cancer stem cells that play a critical role in cancer growth and metastasis. These multifunctional targeted nanoconjugates are also capable of avoiding cancer resistance and monitor predictive molecular changes that open the path for preventive action against pre-cancerous cells, minimizing costs and incidence of relapses. A myriad of nanoconjugates with effective silencing and site-targeting moieties can be developed by incorporating a diverse selection of targeting, diagnostic, and therapeutic components. A discussion of the integrative effort of nanotechnology systems with recent developments of biomolecular interactions in cancer progression is clearly required. Here, we will update the state of the art related to the development and applications of nanoscale platforms and novel biomolecular players in cancer diagnosis, imaging and treatment.

Carlos, Fabio Ferreira, Jose Silva-Nunes, Orfeu Flores, Miguel Brito, Goncalo Doria, Luisa Veiga, and Pedro Viana Baptista. "Association of FTO and PPARG polymorphisms with obesity in Portuguese women." Diabetes, metabolic syndrome and obesity : targets and therapy 6 (2013): 241-5. Abstract

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Veigas, B., P. Pedrosa, I. Couto, M. Viveiros, and P. V. Baptista. "Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis." J Nanobiotechnology 11 (2013): 38. AbstractWebsite

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Machado, Diana, Isabel Couto, João Perdigão, Liliana Rodrigues, Isabel Portugal, Pedro Baptista, Bruno Veigas, Leonard Amaral, and Miguel Viveiros. "Contribution of efflux to the emergence of isoniazid and multidrug resistance in Mycobacterium tuberculosis." PLoS ONE 7 (2012): e34538.
Veigas, Bruno, Jorge M. Jacob, Mafalda N. Costa, David S. Santos, Miguel Viveiros, Joao Inacio, Rodrigo Martins, Pedro Barquinha, Elvira Fortunato, and Pedro Viana Baptista. "Gold on paper-paper platform for Au-nanoprobe TB detection." Lab on a Chip 12 (2012): 4802-4808. Abstract

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Conde, João, Pedro V. Baptista, Yulan Hernández, Vanesa Sanz, and Jesus M. de la Fuente. "Modification of plasmid DNA topology by ‘histone-mimetic’ gold nanoparticles." Nanomedicine (2012).
Baptista, Pedro Viana. "Could gold nanoprobes be an important tool in cancer diagnostics?" Expert Review of Molecular Diagnostics 12 (2012): 541-543. Abstract

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dos Santos, Margarida Moreira, Margarida João Queiroz, and Pedro V. Baptista. "Enhancement of antibiotic effect via gold:silver alloy nanoparticles." J. Nanopar. Res. 14 (2012): 859.
Rosa, J., J. Conde, J. M. de la Fuente, J. C. Lima, and P. V. Baptista. "Gold-nanobeacons for real-time monitoring of RNA synthesis." Biosens Bioelectron 36 (2012): 161-7. AbstractWebsite

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Sanz, Vanesa, João Conde, Yulán Hernández, Pedro V. Baptista, M. R. Ibarra, and Jesús M. de la Fuente. "Effect of PEG biofunctional spacers and TAT peptide on dsRNA loading on gold nanoparticles." J. Nanoparticles Res. 14 (2012): 917.
Conde, Joao, Goncalo Doria, and Pedro Baptista. "Noble metal nanoparticles applications in cancer." Journal of drug delivery 2012 (2012): 751075. Abstract

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Conde, Joao, Alfredo Ambrosone, Vanesa Sanz, Yulan Hernandez, Valentina Marchesano, Furong Tian, Hannah Child, Catherine C. Berry, Ricardo M. Ibarra, Pedro V. Baptista, Claudia Tortiglione, and Jesus M. de la Fuente. "Design of Multifunctional Gold Nanoparticles for In Vitro and In Vivo Gene Silencing." Acs Nano 6 (2012): 8316-8324. Abstract

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Sanz, Vanesa, Joao Conde, Alfredo Ambrosone, Yulan Hernandez, Valentina Marchesasno, Giovani G. Estrada, Manuel R. Ibarra, Pedro V. Baptista, Furong Tian, Claudia Tortiglione, and Jesus M. de la Fuente. "Multifunctional gold nanoparticles for gene silencing." Abstracts of Papers of the American Chemical Society 243 (2012). Abstract

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Guirgis, Bassem S. S., Claudia Sa e Cunha, Ines Gomes, Miguel Cavadas, Isabel Silva, Goncalo Doria, Gregory L. Blatch, Pedro V. Baptista, Eulalia Pereira, Hassan M. E. Azzazy, Maria M. Mota, Miguel Prudencio, and Ricardo Franco. "Gold nanoparticle-based fluorescence immunoassay for malaria antigen detection." Analytical and Bioanalytical Chemistry 402 (2012): 1019-1027. Abstract

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Conde, João, João Rosa, João C. Lima, and Pedro V. Baptista. "Nanophotonics for Molecular Diagnostics and Therapy Applications." Int. J. Photoenergy 619530 (2012).
Doria, G., J. Conde, B. Veigas, L. Giestas, C. Almeida, M. Assunção, J. Rosa, and P. V. Baptista. "Noble metal nanoparticles for biosensing applications." Sensors (Basel) 12 (2012): 1657-87. AbstractWebsite

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Larguinho, Miguel, and Pedro V. Baptista. "Gold and silver nanoparticles for clinical diagnostics - from genomics to proteomics." J. Proteomics 75 (2012): 2811-2823.
Baptista, Pedro V. "RNA quantification with gold nanoprobes for cancer diagnostics." Clinics in Laboratory Medicine 32 (2012): 1-13.
Conde, J., J. Rosa, J. C. Lima, and P. V. Baptista. "Nanophotonics for Molecular Diagnostics and Therapy Applications." International Journal of Photoenergy (2012). Abstract

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