By Type: Book Chapter

Vinhas, Raquel, Alexandra R. Fernandes, and Pedro V. Baptista. "Molecular Diagnostics of Chronic Myeloid Leukemia - precision medicine via gold nanoparticles." In In CDx and precision medicine for hematologic malignancies, edited by Il-Jin Kim, 205-230. PanStanford Publishing, 2018.
Pedrosa, Pedro, and Pedro Viana Baptista. "Gold and silver nanoparticles for diagnostics of infection." In Nanotechnology in Diagnosis, Treatment and Prophylaxis of Infectious Diseases, edited by Mahendra Rai and Kateryna Kon, 1-18. Elsevier, 2015. Abstract

Nanotechnology in Diagnosis, Treatment and Prophylaxis of Infectious Diseases delivers comprehensive coverage of the application of nanotechnology to pressing problems in infectious disease.
This text equips readers with cutting-edge knowledge of promising developments and future prospects in nanotechnology, paying special attention to microbes that are now resistant to conventional antibiotics, a concerning problem in modern medicine.
Readers will find a thorough discussion of this new approach to infectious disease treatment, including the reasons nanotechnology presents a promising avenue for the diagnosis, treatment, and prophylaxis of infectious diseases.

Larguinho, Miguel, Sara Figueiredo, Ana Cordeiro, Fábio Ferreira Carlos, Milton Cordeiro, Pedro Pedrosa, and Pedro Viana Baptista. "Nanoparticles for Diagnostics and Imaging." In Frontiers in Nanomedicine, edited by Maria Luisa Bondì Bondì, Chiara Botto and Erika Amore, 3-46. Bentham Science, 2015. Abstractsample.pdf

Nanoparticles possess unique optical and physic-chemical properties that may potentiate applications in biomedicine, in particular in diagnostics, therapy and imaging. Advances on biomolecular diagnostics strategies have greatly focused on single molecule detection and characterization of DNA, RNA or proteins through improved nanoparticle-based platforms. Nanoparticles improve analytical capability when compared to traditional techniques with high resolution and medium-high throughput. Also, particular interest has been directed at SNP detection, gene expression profiles and biomarker characterization through colorimetric, spectrometric or electrochemical strategies.
Molecular imaging has also benefited from the introduction of nanoparticles in standard techniques towards non-invasive imaging procedures that can be used to highlight regions of interest, allowing the characterization of biological processes at the cellular and/or molecular level. Several imaging modalities are associated with low sensitivity, an issue that can be tackled by the use of probes, e.g. contrast agents for X-ray and magnetic resonance imaging, radiolabelled molecules for nuclear medicine. Furthermore, nanoparticles can be used as vehicles that deliver specifically these contrast agents, leading to overcome the limitations of conventional modalities.
This chapter will discuss the use of nanoparticles in biomolecular recognition and imaging applications, focusing those already being translated into clinical settings. Current knowledge will be addressed as well as its evolution towards the future of nanoparticle-based biomedical applications.

Franco, Ricardo, Pedro Pedrosa, Fábio Ferreira Carlos, Bruno Veigas, and Pedro Viana Baptista. "Gold Nanoparticles for DNA/RNA-Based Diagnostics." In Handbook of Nanoparticles, 1-25. Berlin: Springer, 2015. Abstract

The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted development
in exploring biomolecular interactions with AuNPs-containing systems, pursuing biomedical applications
in diagnostics. Among these applications, AuNPs have been remarkably useful for the development of
DNA/RNA detection and characterization systems for diagnostics, including systems suitable for point of
need. Here, emphasis will be on available molecular detection schemes of relevant pathogens and their
molecular characterization, genomic sequences associated with medical conditions (including cancer),
mutation and polymorphism identification, and the quantification of gene expression.

Baptista, Pedro V., Gonçalo Doria, Pedro Quaresma, Miguel Cavadas, Cristina S. Neves, Inês Gomes, Peter Eaton, Eulália Pereira, and Ricardo Franco. "Nanoparticles in molecular diagnostics. Nanoparticles in Translational Science and Medicine." In Progress in Molecular Biology and Translational Science, edited by A. Villaverde and Michael P. Conn. Elsevier, 2012.
Veigas, Bruno, Gonçalo Doria, and Pedro V. Baptista. "Nanodiagnostics for tuberculosis." In Understanding Tuberculosis - Global Experiences and Innovative Approaches to the Diagnosis, edited by Pere-Joan Cardona. InTech, 2011.