RNA G-quadruplexes (G4s) are abundant and promising drug targets. G-rich RNA forms G4s, while GC-rich sequences form stem-loops, and their structural balance is key to RNA function. Dicer processes stem-loop structures in precursor microRNAs (pre-miRs), but G4s can interfere with this, affecting miR maturation.
Polyetheretherketone (PEEK) is a high-performance thermoplastic that, when combined with Additive Manufacturing (AM), presents considerable advantages in producing customizable implantable medical devices.
We developed three-dimensional cancer models that mimic the interactions in the TME and the tumor structure and complexity is of great relevance to cancer research and drug development.
A simple method for developing ToC devices in under 10 minutes, using thermo-sensitive polystyrene sheets – “Shrinky-dinks”. The process only involves pattern engraving, layer bonding, and heat shrink to the final size.
Novel transfection approach for gene therapy applications in 2D and 3D tumor models based on the use of mild photothermy mediated by AuNPs combined with visible laser irradiation that might pave the way for the spatial-temporal control of gene modulation - 
We use membrane-localized magnetic hyperthermia to promote the internalization of cell-impermeant probes.
Transitioning from 2D to 3D models demands an understanding of altered internalization mechanisms and microenvironmental influences.