Publications in the Year: 2016

Journal Article

Corvo, LM, Mendo AS, Figueiredo S, Gaspar R, Larguinho M, da Silva FGMC, Baptista PV, Fernandes AR.  2016.  Liposomes as Delivery System of a Sn(IV) Complex for Cancer Therapy. Pharmaceutical Research.
Baptista, PV.  2016.  Precision nanomedicine in cancer: how far are we from personalization? Expert Review of Precision Medicine and Drug Development. 1:227-228., Number 3 Abstract

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Mendes, R, Carreira B, Baptista PV, Fernandes AR.  2016.  Non-small cell lung cancer biomarkers and targeted therapy - two faces of the same coin fostered by nanotechnology. Expert Review of Precision Medicine and Drug Development. 1:155-168., Number 2 Abstract

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Cordeiro, M, Carlos FF, Pedrosa P, Lopez A, Baptista PV.  2016.  Gold Nanoparticles for Diagnostics: Advances towards Points of Care. Diagnostics. 6(4):43. AbstractWebsite

The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted developments in the exploration of biomolecular interactions with AuNP-containing systems, in particular for biomedical applications in diagnostics. These systems show great promise in improving sensitivity, ease of operation and portability. Despite this endeavor, most platforms have yet to reach maturity and make their way into clinics or points of care (POC). Here, we present an overview of emerging and available molecular diagnostics using AuNPs for biomedical sensing that are currently being translated to the clinical setting.

Vinhas, R, Cordeiro M, Pedrosa P, Fernandes AR, Baptista PV.  2016.  Current trends in molecular diagnostics of chronic myeloid leukemia. Leukemia & Lymphoma. :1-14. AbstractWebsite

Nearly 1.5 million people worldwide suffer from chronic myeloid leukemia (CML), characterized by the genetic translocation t(9;22)(q34;q11.2), involving the fusion of the Abelson oncogene (ABL1) with the breakpoint cluster region (BCR) gene. Early onset diagnosis coupled to current therapeutics allow for a treatment success rate of 90, which has focused research on the development of novel diagnostics approaches. In this review, we present a critical perspective on current strategies for CML diagnostics, comparing to gold standard methodologies and with an eye on the future trends on nanotheranostics.
Martins, M, Baptista PV, Mendo AS, Correia C, Videira P, Rodrigues AS, Muthukumaran J, Santos-Silva T, Silva A, Guedes da Silva FMC, Gigante J, Duarte A, Gajewska M, Fernandes AR.  2016.  In vitro and in vivo biological characterization of the anti-proliferative potential of a cyclic trinuclear organotin(IV) complex. Molecular Biosystems. 12:1015-1023., Number 3 Abstract

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Vinhas, R, Tolmatcheva A, Canto R, Ribeiro P, Lourenco A, de Sousa AB, Baptista PV, Fernandes AR.  2016.  A novel mutation in CEBPA gene in a patient with acute myeloid leukemia. Leukemia & Lymphoma. 57:711-713., Number 3 Abstract

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Ma, Z, Zhang B, da Silva FGMC, Silva J, Mendo AS, Baptista PV, Fernandes AR.  2016.  Synthesis, Characterization, Thermal Properties and Antiproliferative Potential of Copper(II) 4′-phenyl-terpyridine Compounds. Dalton Transactions. 45(12)
Luisa Corvo, M, Mendo AS, Figueiredo S, Gaspar R, Larguinho M, Guedes da Silva FMC, Baptista PV, Fernandes AR.  2016.  Liposomes as Delivery System of a Sn(IV) Complex for Cancer Therapy. Pharmaceutical Research. 33:1351-1358., Number 6 Abstract

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Vinhas, R, Correia C, Ribeiro P, Lourenco A, de Sousa AB, Fernandes AR, Baptista PV.  2016.  Colorimetric assessment of BCR-ABL1 transcripts in clinical samples via gold nanoprobes. Analytical and Bioanalytical Chemistry. 408:5277-5284., Number 19 Abstract

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Cordeiro, M, Giestes L, Lima JC, Baptista P.  2016.  BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia. Journal of Nanobiotechnology. 38(14) AbstractWebsite

Background
Gold-nanobeacons (Au-nanobeacons) have proven to be versatile systems for molecular diagnostics and therapeutic actuators. Here, we present the development and characterization of two gold nanobeacons combined with Förster resonance energy transfer (FRET) based spectral codification for dual mode sequence discrimination. This is the combination of two powerful technologies onto a single nanosystem.

Results
We proved this concept to detect the most common fusion sequences associated with the development of chronic myeloid leukemia, e13a2 and e14a2. The detection is based on spectral shift of the donor signal to the acceptor, which allows for corroboration of the hybridization event. The Au-nanobeacon acts as scaffold for detection of the target in a homogenous format whose output capability (i.e. additional layer of information) is potentiated via the spectral codification strategy.

Conclusions
The spectral coded Au-nanobeacons permit the detection of each of the pathogenic fusion sequences, with high specificity towards partial complementary sequences. The proposed BioCode Au-nanobeacon concept provides for a nanoplatform for molecular recognition suitable for cancer diagnostics.
Lenis-Rojas, OA, Fernandes AR, Roma-Rodrigues C, Baptista PV, Marques F, Perez-Fernandez D, Guerra-Varela J, Sanchez L, Vazquez-Garcia D, Lopez Torres M, Fernandez A, Fernandez JJ.  2016.  Heteroleptic mononuclear compounds of ruthenium(II): synthesis, structural analyses, in vivo antitumor activity and in vivo toxicity on zebrafish embryost. Dalton Transactions. 45:19127-19140., Number 47 Abstract

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Roma-Rodrigues, C, Heuer-Jungemann A, Fernandes AR, Kanaras AG, Baptista PV.  2016.  Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo. International Journal of Nanomedicine. 11:2633-2639. Abstract

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Conde, J, Tian F, de la Fuente JM, Baptista PV.  2016.  Editorial: Cancer Nanotheranostics: What Have We Learned So Far? Frontiers in Chemistry. 71(3)Website
Cordeiro, M, Carlos FF, Pedrosa P, Lopez A, Baptista PV.  2016.  Gold Nanoparticles for Diagnostics: Advances towards Points of Care. Diagnostics. 6, Number 4 Abstract

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Roma-Rodrigues, C, Alves-Barroco C, Raposo LR, Costa MN, Fortunato E, Baptista PV, Fernandes AR, Santos-Sanches I.  2016.  Infection of human keratinocytes by Streptococcus dysgalactiae subspecies dysgalactiae isolated from milk of the bovine udder. Microbes and Infection. 18:290-293., Number 4 Abstract

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