By Type: Journal Article

Nanoparticles—Emerging Potential for Managing Leukemia and Lymphoma, Vinhas, Raquel, Mendes Rita, Fernandes Alexandra R., and Baptista Pedro V. , Front. Bioeng. Biotechnol, 2017, Volume 5, p.79, (2017) AbstractWebsite

Nanotechnology has become a powerful approach to improve the way we diagnose and treat cancer. In particular, nanoparticles possess unique features for enhanced sensitivity and selectivity for earlier detection of circulating cancer biomarkers. In vivo, nanoparticles enhance the therapeutic efficacy of anticancer agents when compared to conventional chemotherapy, improving vectorization and delivery, and helping to overcome drug resistance. Nanomedicine has been mostly focused on solid cancers due to take advantage from the enhanced permeability and retention (EPR) effect experienced by tissues in the close vicinity of tumors, which enhance nanomedicine’s accumulation and, consequently, improve efficacy. Nanomedicines for leukemia and lymphoma, where EPR effect is not a factor, are addressed differently from solid tumors. Nevertheless, nanoparticles have provided innovative approaches to simple and non-invasive methodologies for diagnosis and treatment in liquid tumors. In this review, we consider the state of the art on different types of nanoconstructs for the management of liquid tumors, from pre-clinical studies to clinical trials. We also discuss the advantages of nanoplatforms for theranostics and the central role played by nanoparticles in this combined strategy.

Digital Microfluidics for Nucleic Acid Amplification, Coelho, Beatriz, Veigas Bruno, Fortunato Elvira, Martins Rodrigo, Aguas Hugo, Igreja Rui, and Baptista Pedro V. , Sensors, Volume 17, Number 7, (2017) Abstract

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Potentiating angiogenesis arrest in vivo via laser irradiation of peptide functionalised gold nanoparticles, Pedrosa, Pedro, Heuer-Jungemann Amelie, Kanaras Antonios G., Fernandes Alexandra R., and Baptista Pedro V. , Journal of Nanobiotechnology, Volume 15, (2017) Abstract

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Liposomes as Delivery System of a Sn(IV) Complex for Cancer Therapy, Corvo, Luísa M., Mendo Ana Soraia, Figueiredo Sara, Gaspar Rogério, Larguinho Miguel, da Silva Fátima Guedes M. C., Baptista Pedro Viana, and Fernandes Alexandra R. , Pharmaceutical Research, (2016)
Synthesis, Characterization, Thermal Properties and Antiproliferative Potential of Copper(II) 4′-phenyl-terpyridine Compounds, Ma, Zhen, Zhang Bian, da Silva Fátima Guedes M. C., Silva Joana, Mendo Ana Soraia, Baptista Pedro Viana, and Fernandes Alexandra R. , Dalton Transactions, Volume 45, Issue 12, (2016)
Heteroleptic mononuclear compounds of ruthenium(II): synthesis, structural analyses, in vivo antitumor activity and in vivo toxicity on zebrafish embryost, Lenis-Rojas, O. A., Fernandes A. R., Roma-Rodrigues C., Baptista P. V., Marques F., Perez-Fernandez D., Guerra-Varela J., Sanchez L., Vazquez-Garcia D., Lopez Torres M., Fernandez A., and Fernandez J. J. , Dalton Transactions, Volume 45, Number 47, p.19127-19140, (2016) Abstract

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Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo, Roma-Rodrigues, Catarina, Heuer-Jungemann Amelie, Fernandes Alexandra R., Kanaras Antonios G., and Baptista Pedro V. , International Journal of Nanomedicine, Volume 11, p.2633-2639, (2016) Abstract

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BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia, Cordeiro, Milton, Giestes Leticia, Lima Joao Carlos, and Baptista Pedro , Journal of Nanobiotechnology, Volume 38, Issue 14, (2016) AbstractWebsite

Background
Gold-nanobeacons (Au-nanobeacons) have proven to be versatile systems for molecular diagnostics and therapeutic actuators. Here, we present the development and characterization of two gold nanobeacons combined with Förster resonance energy transfer (FRET) based spectral codification for dual mode sequence discrimination. This is the combination of two powerful technologies onto a single nanosystem.

Results
We proved this concept to detect the most common fusion sequences associated with the development of chronic myeloid leukemia, e13a2 and e14a2. The detection is based on spectral shift of the donor signal to the acceptor, which allows for corroboration of the hybridization event. The Au-nanobeacon acts as scaffold for detection of the target in a homogenous format whose output capability (i.e. additional layer of information) is potentiated via the spectral codification strategy.

Conclusions
The spectral coded Au-nanobeacons permit the detection of each of the pathogenic fusion sequences, with high specificity towards partial complementary sequences. The proposed BioCode Au-nanobeacon concept provides for a nanoplatform for molecular recognition suitable for cancer diagnostics.

Precision nanomedicine in cancer: how far are we from personalization?, Baptista, Pedro Viana , Expert Review of Precision Medicine and Drug Development, Volume 1, Number 3, p.227-228, (2016) Abstract

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Gold Nanoparticles for Diagnostics: Advances towards Points of Care, Cordeiro, Mílton, Carlos Fábio Ferreira, Pedrosa Pedro, Lopez António, and Baptista Pedro Viana , Diagnostics, Volume 6, Issue 4, p.43, (2016) AbstractWebsite

The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted developments in the exploration of biomolecular interactions with AuNP-containing systems, in particular for biomedical applications in diagnostics. These systems show great promise in improving sensitivity, ease of operation and portability. Despite this endeavor, most platforms have yet to reach maturity and make their way into clinics or points of care (POC). Here, we present an overview of emerging and available molecular diagnostics using AuNPs for biomedical sensing that are currently being translated to the clinical setting.

Liposomes as Delivery System of a Sn(IV) Complex for Cancer Therapy, Luisa Corvo, M., Mendo Ana Soraia, Figueiredo Sara, Gaspar Rogerio, Larguinho Miguel, Guedes da Silva Fatima M. C., Baptista Pedro Viana, and Fernandes Alexandra R. , Pharmaceutical Research, Volume 33, Number 6, p.1351-1358, (2016) Abstract

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Current trends in molecular diagnostics of chronic myeloid leukemia, Vinhas, Raquel, Cordeiro Milton, Pedrosa Pedro, Fernandes Alexandra R., and Baptista Pedro V. , Leukemia & Lymphoma, p.1-14, (2016) AbstractWebsite

Nearly 1.5 million people worldwide suffer from chronic myeloid leukemia (CML), characterized by the genetic translocation t(9;22)(q34;q11.2), involving the fusion of the Abelson oncogene (ABL1) with the breakpoint cluster region (BCR) gene. Early onset diagnosis coupled to current therapeutics allow for a treatment success rate of 90, which has focused research on the development of novel diagnostics approaches. In this review, we present a critical perspective on current strategies for CML diagnostics, comparing to gold standard methodologies and with an eye on the future trends on nanotheranostics.

In vitro and in vivo biological characterization of the anti-proliferative potential of a cyclic trinuclear organotin(IV) complex, Martins, Marta, Baptista Pedro V., Mendo Ana Soraia, Correia Claudia, Videira Paula, Rodrigues Antonio S., Muthukumaran J., Santos-Silva Teresa, Silva Ana, Guedes da Silva Fatima M. C., Gigante Joana, Duarte Antonio, Gajewska Malgorzata, and Fernandes Alexandra R. , Molecular Biosystems, Volume 12, Number 3, p.1015-1023, (2016) Abstract

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Colorimetric assessment of BCR-ABL1 transcripts in clinical samples via gold nanoprobes, Vinhas, Raquel, Correia Claudia, Ribeiro Patricia, Lourenco Alexandra, de Sousa Aida Botelho, Fernandes Alexandra R., and Baptista Pedro V. , Analytical and Bioanalytical Chemistry, Volume 408, Number 19, p.5277-5284, (2016) Abstract

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Gold Nanoparticles for Diagnostics: Advances towards Points of Care, Cordeiro, Milton, Carlos Fabio Ferreira, Pedrosa Pedro, Lopez Antonio, and Baptista Pedro Viana , Diagnostics, Volume 6, Number 4, (2016) Abstract

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Non-small cell lung cancer biomarkers and targeted therapy - two faces of the same coin fostered by nanotechnology, Mendes, Rita, Carreira Barbara, Baptista Pedro V., and Fernandes Alexandra R. , Expert Review of Precision Medicine and Drug Development, Volume 1, Number 2, p.155-168, (2016) Abstract

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A novel mutation in CEBPA gene in a patient with acute myeloid leukemia, Vinhas, Raquel, Tolmatcheva Anna, Canto Rafaela, Ribeiro Patricia, Lourenco Alexandra, de Sousa Aida Botelho, Baptista Pedro V., and Fernandes Alexandra R. , Leukemia & Lymphoma, Volume 57, Number 3, p.711-713, (2016) Abstract

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Editorial: Cancer Nanotheranostics: What Have We Learned So Far?, Conde, Joao, Tian Furong, de la Fuente Jesus M., and Baptista Pedro V. , Frontiers in Chemistry, Volume 71, Issue 3, (2016) Website
Infection of human keratinocytes by Streptococcus dysgalactiae subspecies dysgalactiae isolated from milk of the bovine udder, Roma-Rodrigues, Catarina, Alves-Barroco Cynthia, Raposo Luis R., Costa Mafalda N., Fortunato Elvira, Baptista Pedro Viana, Fernandes Alexandra R., and Santos-Sanches Ilda , Microbes and Infection, Volume 18, Number 4, p.290-293, (2016) Abstract

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Gold nanoparticle-based theranostics: disease diagnostics and treatment using a single nanomaterial, Vinhas, Raquel, Cordeiro Milton, Carlos Fábio, Mendo Soraia, Fernandes Alexandra, Figueiredo Sara, and Baptista Pedro , Journal of Nanobiosensors in Disease Diagnosis, Issue 4, p.11-23, (2015) AbstractWebsite

Nanotheranostics takes advantage of nanotechnology-based systems in order to diagnose and treat a specific disease. This approach is particularly relevant for personalized medicine, allowing the detection of a disease at an early stage, to direct a suitable therapy toward the target tissue based on the molecular profile of the altered phenotype, subsequently facilitating disease monitoring and following treatment. A tailored strategy also enables to reduce the off-target effects associated with universal treatments and improve the safety profile of a given treatment. The unique optical properties of gold nanoparticles, their ease of surface modification, and high surface-to-volume ratio have made them central players in this area. By combining imaging, targeting, and therapeutic agents in a single vehicle, these nanoconjugates are (ought to be) an important tool in the clinics. In this review, the multifunctionality of gold nanoparticles as theranostics agents will be highlighted, as well as the requirements before the translation of these nanoplatforms into routine clinical practice.

DNA adduct identification using gold-aptamer nanoprobes, Larguinho, Miguel, Santos Sofia, Almeida Joao, and Baptista Pedro V. , Iet Nanobiotechnology, Volume 9, Number 2, p.95-101, (2015) Abstract

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Field Effect Sensors for Nucleic Acid Detection: Recent Advances and Future Perspectives, Veigas, Bruno, Fortunato Elvira, and Baptista Pedro V. , Sensors, Volume 15, Number 5, p.10380-10398, (2015) Abstract

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Organometallic Compounds in Cancer Therapy: Past Lessons and Future Directions, Martins, Pedro, Marques Mara, Coito Lidia, Pombeiro Armando, Baptista Pedro V., and Fernandes Alexandra R. , ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, Volume 9, Issue 14, (2015) AbstractWebsite

Over the past few years, modern medicinal chemistry has evolved towards providing us new and alternative chemotherapeutic compounds with high cytotoxicity towards tumor cells, alongside with reduced side effects in cancer patients. Organometallic compounds and their unique physic-chemical properties typically used in homogenous catalysis are now being translated as potential candidates for medical purposes. Their structural diversity, ligand exchange, redox and catalytic properties make them promising drug candidates for cancer therapy. Over the last decade this area has witnessed a steady growth and a few organometallic compounds have in fact already entered clinical trials, emphasizing its increasing importance and clinical relevance. Here we intend to stress out the different applications of organometallic compounds in medicine with emphasis on cancer therapy, as well as address setbacks regarding formulation issues, systemic toxicity and off-target effects. Advantages over classical coordination metal complexes, their nanovectorisation and specific molecular targets are also discussed.

One nanoprobe, two pathogens: gold nanoprobes multiplexing for point-of-care, Veigas, Bruno, Pedrosa Pedro, Carlos Fabio F., Mancio-Silva Liliana, Grosso Ana Rita, Fortunato Elvira, Mota Maria M., and Baptista Pedro V. , Journal of Nanobiotechnology, Volume 13, (2015) Abstract

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Single Nucleotide Polymorphism Detection Using Gold Nanoprobes and Bio-Microfluidic Platform With Embedded Micro lenses, Bernacka-Wojcik, Iwona, Aguas Hugo, Carlos Fabio Ferreira, Lopes Paulo, Wojcik Pawel Jerzy, Costa Mafalda Nascimento, Veigas Bruno, Igreja Rui, Fortunato Elvira, Baptista Pedro Viana, and Martins Rodrigo , Biotechnology and Bioengineering, Volume 112, Number 6, p.1210-1219, (2015) Abstract

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