Citation:

RNAi-based glyconanoparticles trigger apoptotic pathways for in vitro and in vivo enhanced cancer-cell killing, Conde, João, Tian Furong, Hernandez Yulan, Bao Chenchen, Baptista {Pedro Miguel Ribeiro Viana}, Cui Daxiang, Stoeger Tobias, and {de la Fuente} {Jesus M. } , Nanoscale, Volume 7, Number 19, p.9083–9091, (2015)

Abstract:

Gold glyconanoparticles (GlycoNPs) are full of promise in areas like biomedicine, biotechnology and materials science due to their amazing physical, chemical and biological properties. Here, siRNA GlycoNPs (AuNP@PEG@Glucose@siRNA) in comparison with PEGylated GlycoNPs (AuNP@PEG@Glucose) were applied in vitro to a luciferase-CMT/167 adenocarcinoma cancer cell line and in vivo via intratracheal instillation directly into the lungs of B6 albino mice grafted with luciferase-CMT/167 adenocarcinoma cells. siRNA GlycoNPs but not PEGylated GlycoNPs induced the expression of pro-apoptotic proteins such as Fas/CD95 and caspases 3 and 9 in CMT/167 adenocarcinoma cells in a dose dependent manner, independent of the inflammatory response, evaluated by bronchoalveolar lavage cell counting. Moreover, in vivo pulmonary delivered siRNA GlycoNPs were capable of targeting c-Myc gene expression (a crucial regulator of cell proliferation and apoptosis) via in vivo RNAi in tumour tissue, leading to an similar to 80% reduction in tumour size without associated inflammation.

Notes:

The authors thank the ERANET-NANOSCIERA NANOTRUCK project for financial support. JMF thanks Fondo Social Europeo for financial support.