Haque, S, Alexandre M, Baretzky C, Rossi D, Rossi FD, Vicente AT, Brunetti F, Águas H, Ferreira RAS, Fortunato E, Maur MAD, Wurfel U, Martins R, Mendes MJ.
2022.
Photonic-Structured Perovskite Solar Cells: Detailed Optoelectronic Analysis. ACS Photonics. 9(7):2408–2421.
Ramos, A, Isufi B, Marreiros R, Bolesova M, Gajdsova K.
2022.
Rational Use of FPFRC in Slab-Connections Under Reversed Horizontal Cyclic Loading. Engineering Structures. Accepted for publication
AbstractSlab – column connections that are subjected to combined gravity and horizontal loading during an earthquake are prone to premature failure due to punching shear. Traditional solutions to avoid punching failure and to increase the displacement capacity of this type of connection include using stirrups and double-headed studs as shear reinforcement. The use of High-Performance Fiber Reinforced Concrete (HPFRC) in a small region of the slab around the column as a substitute for traditional solutions is investigated in this paper, because this material has the potential to reduce labor and material costs. To fulfill this objective, four slab specimens with a thickness of 150 mm were tested under combined gravity and reversed horizontal drifts. The results are discussed in detail. The experimental variables considered were the top flexural reinforcement ratio, the size of the HPFRC zone and the intensity of the gravity load. Previously published tests that serve as reference specimens are used to compare the results. The behavior of the specimens with HPFRC was substantially improved compared to the reference specimens in terms of drift capacity: from only 1.0% drift to above 5.5%, even though a very small quantity of HPFRC was used, extended up to only 1.5 times the effective depth of the slab from the face of the column. Specimens with HPFRC also behaved better when compared to specimens with High-Strength Concrete (HSC). Side effects of using HPFRC in the slab in the vicinity of the column include an increase of the unbalanced moment transfer capacity and lateral stiffness, as well as a reduction of the deflections of the slab.
Karamash, M, Stumpe M, Dengjel J, Salgueiro CA, Giese B, Fromm KM.
2022.
Reduction Kinetic of Water Soluble Metal Salts by Geobacter sulfurreducens: Fe2+/Hemes Stabilize and Regulate Electron Flux Rates. Frontiers in Microbiology. 13
AbstractGeobacter sulfurreducens is a widely applied microorganism for the reduction of toxic metal salts, as an electron source for bioelectrochemical devices, and as a reagent for the synthesis of nanoparticles. In order to understand the influence of metal salts, and of electron transporting, multiheme c-cytochromes on the electron flux during respiration of G. sulfurreducens, the reduction kinetic of Fe3+, Co3+, V5+, Cr6+, and Mn7+ containing complexes were measured. Starting from the resting phase, each G. sulfurreducens cell produced an electron flux of 3.7 × 105 electrons per second during the respiration process. Reduction rates were within ± 30% the same for the 6 different metal salts, and reaction kinetics were of zero order. Decrease of c-cytochrome concentrations by downregulation and mutation demonstrated that c-cytochromes stabilized respiration rates by variation of their redox states. Increasing Fe2+/heme levels increased electron flux rates, and induced respiration flexibility. The kinetic effects parallel electrochemical results of G. sulfurreducens biofilms on electrodes, and might help to optimize bioelectrochemical devices.
Gonçalves, AM, Sousa Â, Pedro AQ, Romão MJ, Queiroz JA, Gallardo E, Passarinha LA.
2022.
Advances in Membrane-Bound Catechol-O-Methyltransferase Stability Achieved Using a New Ionic Liquid-Based Storage Formulation. International Journal of Molecular Sciences. 23, Number 13
AbstractMembrane-bound catechol-O-methyltransferase (MBCOMT), present in the brain and involved in the main pathway of the catechol neurotransmitter deactivation, is linked to several types of human dementia, which are relevant pharmacological targets for new potent and nontoxic inhibitors that have been developed, particularly for Parkinson’s disease treatment. However, the inexistence of an MBCOMT 3D-structure presents a blockage in new drugs’ design and clinical studies due to its instability. The enzyme has a clear tendency to lose its biological activity in a short period of time. To avoid the enzyme sequestering into a non-native state during the downstream processing, a multi-component buffer plays a major role, with the addition of additives such as cysteine, glycerol, and trehalose showing promising results towards minimizing hMBCOMT damage and enhancing its stability. In addition, ionic liquids, due to their virtually unlimited choices for cation/anion paring, are potential protein stabilizers for the process and storage buffers. Screening experiments were designed to evaluate the effect of distinct cation/anion ILs interaction in hMBCOMT enzymatic activity. The ionic liquids: choline glutamate [Ch][Glu], choline dihydrogen phosphate ([Ch][DHP]), choline chloride ([Ch]Cl), 1- dodecyl-3-methylimidazolium chloride ([C12mim]Cl), and 1-butyl-3-methylimidazolium chloride ([C4mim]Cl) were supplemented to hMBCOMT lysates in a concentration from 5 to 500 mM. A major potential stabilizing effect was obtained using [Ch][DHP] (10 and 50 mM). From the DoE 146% of hMBCOMT activity recovery was obtained with [Ch][DHP] optimal conditions (7.5 mM) at −80 °C during 32.4 h. These results are of crucial importance for further drug development once the enzyme can be stabilized for longer periods of time.