Task 1. Polyphenolic characterization

 Polyphenolic characterization of selected natural extracts
The first goal is the polyphenolic characterization of myrtle (Myrtus communis), pomegranate peel (Punica granatum) and galls (Quercus infectoria and portuguese galls). Several extraction procedures based on Iberian technical texts will be tested. Furthermore, different polyphenols will be identified and
isolated as isomers and individual compounds. Compound isolation and purification will be achieved using Preparative-HPLC-DAD chromatography.
High performance liquid chromatography coupled to mass spectrometry (HPLC-MS) has been widely used in the structural characterization of complex plant extracts and has proved to be an important tool to identify phenolic compounds. Electrospray (ESI) is a soft ionization technique, and tandem mass spectrometry (MS/MS) with ion trap can provide additional information about the structures of polyphenols through the fragmentation patterns of the pseudomolecular ions. Polyphenolic characterization will be achieved by HPLC-DAD, HPLCESI-MS and NMR.
Polyphenols from each source will be selected, isolated and used for the study of the influence of different additives on the reproduction of iron gall inks from Iberian treatises in task 3. They will also be used for the characterization of iron-tannin complexes in task 4.

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TEAM MEMBERS

Natércia Teixeira
(Task Coordinator)

Victor de Freitas
(Task Co-Coordinator)

Maria João Melo

Paula Nabais

André da Silva

Luís Silva

Maria Conceição Rangel

Fernando Pina