Publications

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Structural characterization and biological properties of silver(I) tris(pyrazolyl)methane sulfonate, Almeida, J., Roma-Rodrigues C., Mahmoud A. G., Guedes da Silva M. F. C., Pombeiro A. J. L., Martins D.RS L. M., Baptista P. V., and Fernandes A. R. , J Inorg Biochem, Volume 199, p.110789, (2019) AbstractWebsite

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Multifunctional microfluidic chip for optical nanoprobe based RNA detection – application to Chronic Myeloid Leukemia, Alves, Pedro Urbano, Vinhas Raquel, Fernandes Alexandra R., Birol Semra Zuhal, Trabzon Levent, Bernacka-Wojcik Iwona, Igreja Rui, Lopes Paulo, Baptista Pedro Viana, Águas Hugo, Fortunato Elvira, and Martins Rodrigo , Scientific Reports, 2018, Volume 8, Issue 1, p.381, (2018) AbstractWebsite

Many diseases have their treatment options narrowed and end up being fatal if detected during later stages. As a consequence, point-of-care devices have an increasing importance for routine screening applications in the health sector due to their portability, fast analyses and decreased cost. For that purpose, a multifunctional chip was developed and tested using gold nanoprobes to perform RNA optical detection inside a microfluidic chip without the need of molecular amplification steps. As a proof-of-concept, this device was used for the rapid detection of chronic myeloid leukemia, a hemato-oncological disease that would benefit from early stage diagnostics and screening tests. The chip passively mixed target RNA from samples, gold nanoprobes and saline solution to infer a result from their final colorimetric properties. An optical fiber network was used to evaluate its transmitted spectra inside the chip. Trials provided accurate output results within 3 min, yielding signal-to-noise ratios up to 9 dB. When compared to actual state-of-art screening techniques of chronic myeloid leukemia, these results were, at microscale, at least 10 times faster than the reported detection methods for chronic myeloid leukemia. Concerning point-of-care applications, this work paves the way for other new and more complex versions of optical based genosensors.

Tackling Multidrug Resistance in Streptococci - From Novel Biotherapeutic Strategies to Nanomedicines, Alves-Barroco, C., Rivas-García L., Fernandes A. R., and Baptista P. V. , Front Microbiol, Volume 11, p.579916, (2020) AbstractWebsite

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A promising road with challenges: where are gold nanoparticles in translational research?, Bao, Chenchen, Conde Joao, Polo Ester, del Pino Pablo, Moros Maria, Baptista Pedro V., Grazu Valeria, Cui Daxiang, and de la Fuente Jesus M. , Nanomedicine, Volume 9, Number 15, p.2353-2370, (2014) Abstract

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3h Gold nanobeacons: a potential nanotheranostics platform, Baptista, Pedro Viana , Nanomedicine, Volume 9, Number 15, p.2247-2250, (2014) Abstract

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Nanoparticles in molecular diagnostics, Baptista, P. V., Doria G., Quaresma P., Cavadas M., Neves C. S., Gomes I., Eaton P., Pereira E., and Franco R. , Prog Mol Biol Transl Sci, Volume 104, p.427-88, (2011) AbstractWebsite

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Gold nanoparticles for the development of clinical diagnosis methods, Baptista, P., Pereira E., Eaton P., Doria G., Miranda A., Gomes I., Quaresma P., and Franco R. , Anal Bioanal Chem, Volume 391, Number 3, p.943-50, (2008) AbstractWebsite

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Nanodiagnostics: leaving the research lab to enter the clinics?, Baptista, Pedro V. , Diagnosis, Volume 1, Issue 4, p.305–309, (2014) AbstractWebsite

Nanotechnology has provided a plethora of valuable tools that can be applied for the detection of biomolecules and analytes relevant for diagnosis purposes – nanodiagnostics. This surging new field of molecular diagnostics has been revolutionizing laboratory procedures and providing new ways to assess disease biomarkers with increased sensitivity. While most of the reported nanodiagnostics systems are proof-of-concepts that demonstrate their efficacy in the lab, several nanodiagnostics platforms have already matured to a level that open the way for effective translation to the clinics. Nanodiagnostics platforms (e.g., gold nanoparticles containing systems) have been remarkably useful for the development of molecular diagnosis strategies for DNA/RNA detection and characterization, including systems suitable for point-of-care. How near are nanodiagnostics to go from the bench to the bedside?

Nanoparticles in molecular diagnostics, Baptista, P. V., Doria G., Quaresma P., Cavadas M., Neves C. S., Gomes I., Eaton P., Pereira E., and Franco R. , Prog. Mol. Biol. Transl. Sci., Volume 104, p.427-488, (2011)
Alloy metal nanoparticles for multicolor cancer diagnostics, Baptista, Pedro V., Doria Gonçalo, and Conde João , Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI, San Francisco, CA, USA, (2011)
Precision nanomedicine in cancer: how far are we from personalization?, Baptista, Pedro Viana , Expert Review of Precision Medicine and Drug Development, Volume 1, Number 3, p.227-228, (2016) Abstract

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Direct detection of mutations/SNPs using DNA-derivatised gold nanoparticles, Baptista, P., Doria G., Matias A., Osorio L., Pereira E., and Ricardo F. , Congress of Clinical Chemistry and Laboratory Medicine, Jena, Germany, (2005)
Principles in genetic risk assessment, Baptista, Pedro Viana , THERAPEUTICS AND CLINICAL RISK MANAGEMENT, Volume 1, Issue 1, p.15-20, (2005) AbstractWebsite

Risk assessment constitutes an essential component of genetic counseling and testing, and the genetic risk should be estimated as accurately as possible for individual and family decision making. All relevant information retrieved from population studies and pedigree and genetic testing enhances the accuracy of the assessment of an individual’s genetic risk. This review will focus on the following general aspects implicated in risk assessment: the increasing genetic information regarding disease; complex traits versus Mendelian disorders; and the influence of the environment and disease susceptibility. The influence of these factors on risk assessment will be discussed.

Nanoparticles in Molecular Diagnostics, Baptista, Pedro V., Doria Goncalo, Quaresma Pedro, Cavadas Miguel, Neves Cristina S., Gomes Ines, Eaton Peter, Pereira Eulalia, Franco Ricardo, and Villaverde A. , Nanoparticles in Translational Science and Medicine, Volume 104, p.427-488, (2011) Abstract

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Nanoparticles in molecular diagnostics. Nanoparticles in Translational Science and Medicine, Baptista, Pedro V., Doria Gonçalo, Quaresma Pedro, Cavadas Miguel, Neves Cristina S., Gomes Inês, Eaton Peter, Pereira Eulália, and Franco Ricardo , Progress in Molecular Biology and Translational Science, (2012)
Nanodiagnostics: leaving the research lab to enter the clinics?, Baptista, P. V. , Diagnosis (Berl), Volume 1, Number 4, p.305-309, (2014) AbstractWebsite

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Cancer Nanotechnology - Prospects for Cancer Diagnostics and Therapy, Baptista, Pedro Viana , Current Cancer Therapy Reviews, Volume 5, p.80-88, (2009)
Gold nanobeacons: A potential nanotheranostics platform, Baptista, Pedro Viana , Nanomedicine, Volume 9, Issue 15, p.2247-50, (2014) Website
Colorimetric detection of eukaryotic gene expression with DNA-derivatized gold nanoparticles, Baptista, P., Doria G., Henriques D., Pereira E., and Franco R. , Journal of Biotechnology, Volume 119, Number 2, p.111-117, (2005) Abstract

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Gold Nanoparticles for the Development of Clinical Diagnosis Methods, Baptista, Pedro, Pereira Eulália, Eaton Peter, Doria Gonçalo, Miranda Adelaide, Gomes Inês, Quaresma Pedro, and Franco Ricardo , Anal Bioanal Chem., Volume 391, Issue 3, p.943-950, (2008)
Nano-Strategies to Fight Multidrug Resistant Bacteria—“A Battle of the Titans”, Baptista, Pedro V., McCusker Matthew P., Carvalho Andreia, Ferreira Daniela A., Mohan Niamh M., Martins Marta, and Fernandes Alexandra R. , Frontiers in Microbiology, Volume 9, p.1441, (2018) AbstractWebsite

Infectious diseases remain one of the leading causes of morbidity and mortality worldwide. The WHO and CDC have expressed serious concern regarding the continued increase in the development of multidrug resistance among bacteria. Therefore, the antibiotic resistance crisis is one of the most pressing issues in global public health. Associated with the rise in antibiotic resistance is the lack of new antimicrobials. This has triggered initiatives worldwide to develop novel and more effective antimicrobial compounds as well as to develop novel delivery and targeting strategies. Bacteria have developed many ways by which they become resistant to antimicrobials. Among those are enzyme inactivation, decreased cell permeability, target protection, target overproduction, altered target site/enzyme, increased efflux due to over-expression of efflux pumps, among others. Other more complex phenotypes, such as biofilm formation and quorum sensing do not appear as a result of the exposure of bacteria to antibiotics although, it is known that biofilm formation can be induced by antibiotics. These phenotypes are related to tolerance to antibiotics in bacteria. Different strategies, such as the use of nanostructured materials, are being developed to overcome these and other types of resistance. Nanostructured materials can be used to convey antimicrobials, to assist in the delivery of novel drugs or ultimately, possess antimicrobial activity by themselves. Additionally, nanoparticles (e.g., metallic, organic, carbon nanotubes, etc.) may circumvent drug resistance mechanisms in bacteria and, associated with their antimicrobial potential, inhibit biofilm formation or other important processes. Other strategies, including the combined use of plant-based antimicrobials and nanoparticles to overcome toxicity issues, are also being investigated. Coupling nanoparticles and natural-based antimicrobials (or other repurposed compounds) to inhibit the activity of bacterial efflux pumps; formation of biofilms; interference of quorum sensing; and possibly plasmid curing, are just some of the strategies to combat multidrug resistant bacteria. However, the use of nanoparticles still presents a challenge to therapy and much more research is needed in order to overcome this. In this review, we will summarize the current research on nanoparticles and other nanomaterials and how these are or can be applied in the future to fight multidrug resistant bacteria.

Gold-nanoparticle-probe-based assay for rapid and direct detection of Mycobacterium tuberculosis DNA in clinical samples, Baptista, P. V., Koziol-Montewka M., Paluch-Oles J., and al.et , CLINICAL CHEMISTRY, Volume 52, Number 7, p.1433-1434, (2006) Abstract

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Could gold nanoprobes be an important tool in cancer diagnostics?, Baptista, Pedro Viana , Expert Review of Molecular Diagnostics, Volume 12, Number 6, p.541-543, (2012) Abstract

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Gold nanoprobe-based non-crosslinking hybridization for molecular diagnostics: an update, Baptista, P. V. , Expert Rev Mol Diagn, Volume 18, Number 9, p.767-773, (2018) AbstractWebsite

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Gold nanoparticles for the development of clinical diagnosis methods, Baptista, P., Pereira E., Eaton P., and al.et , ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Volume 391, Number 3, p.943-950, (2008) Abstract

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