Publications

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2017
Photothermal enhancement of chemotherapy in breast cancer by visible irradiation of Gold Nanoparticles, Mendes, Rita, Pedrosa Pedro, Lima Joao C., Fernandes Alexandra R., and Baptista Pedro V. , Scientific Reports, Volume 7, (2017) Abstract

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Potentiating angiogenesis arrest in vivo via laser irradiation of peptide functionalised gold nanoparticles, Pedrosa, Pedro, Heuer-Jungemann Amelie, Kanaras Antonios G., Fernandes Alexandra R., and Baptista Pedro V. , Journal of Nanobiotechnology, Volume 15, (2017) Abstract

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Quantitative real-time monitoring of RCA amplification of cancer biomarkers mediated by a flexible ion sensitive platform, Veigas, Bruno, Pinto Joana, Vinhas Raquel, Calmeiro Tomas, Martins Rodrigo, Fortunato Elvira, and Baptista Pedro Viana , Biosensors & Bioelectronics, Volume 91, p.788-795, (2017) Abstract

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Smuggling gold nanoparticles across cell types - A new role for exosomes in gene silencing, Roma-Rodrigues, Catarina, Pereira Francisca, Alves de Matos Antonio P., Fernandes Marta, Baptista Pedro V., and Fernandes Alexandra R. , Nanomedicine-Nanotechnology Biology and Medicine, Volume 13, Number 4, p.1389-1398, (2017) Abstract

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Targeting canine mammary tumours via gold nanoparticles functionalized with promising Co(II) and Zn(II) compounds, Raposo, L. R., Roma-Rodrigues C., Jesus J., Martins L. M. D. R. S., Pombeiro A. J., Baptista P. V., and Fernandes A. R. , Veterinary and Comparative Oncology, Volume 15, Number 4, p.1537-1542, (2017) Abstract

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2016
BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia, Cordeiro, Milton, Giestes Leticia, Lima Joao Carlos, and Baptista Pedro , Journal of Nanobiotechnology, Volume 38, Issue 14, (2016) AbstractWebsite

Background
Gold-nanobeacons (Au-nanobeacons) have proven to be versatile systems for molecular diagnostics and therapeutic actuators. Here, we present the development and characterization of two gold nanobeacons combined with Förster resonance energy transfer (FRET) based spectral codification for dual mode sequence discrimination. This is the combination of two powerful technologies onto a single nanosystem.

Results
We proved this concept to detect the most common fusion sequences associated with the development of chronic myeloid leukemia, e13a2 and e14a2. The detection is based on spectral shift of the donor signal to the acceptor, which allows for corroboration of the hybridization event. The Au-nanobeacon acts as scaffold for detection of the target in a homogenous format whose output capability (i.e. additional layer of information) is potentiated via the spectral codification strategy.

Conclusions
The spectral coded Au-nanobeacons permit the detection of each of the pathogenic fusion sequences, with high specificity towards partial complementary sequences. The proposed BioCode Au-nanobeacon concept provides for a nanoplatform for molecular recognition suitable for cancer diagnostics.

Current trends in molecular diagnostics of chronic myeloid leukemia, Vinhas, Raquel, Cordeiro Milton, Pedrosa Pedro, Fernandes Alexandra R., and Baptista Pedro V. , Leukemia & Lymphoma, p.1-14, (2016) AbstractWebsite

Nearly 1.5 million people worldwide suffer from chronic myeloid leukemia (CML), characterized by the genetic translocation t(9;22)(q34;q11.2), involving the fusion of the Abelson oncogene (ABL1) with the breakpoint cluster region (BCR) gene. Early onset diagnosis coupled to current therapeutics allow for a treatment success rate of 90, which has focused research on the development of novel diagnostics approaches. In this review, we present a critical perspective on current strategies for CML diagnostics, comparing to gold standard methodologies and with an eye on the future trends on nanotheranostics.

Editorial: Cancer Nanotheranostics: What Have We Learned So Far?, Conde, Joao, Tian Furong, de la Fuente Jesus M., and Baptista Pedro V. , Frontiers in Chemistry, Volume 71, Issue 3, (2016) Website
Gold Nanoparticles for Diagnostics: Advances towards Points of Care, Cordeiro, Mílton, Carlos Fábio Ferreira, Pedrosa Pedro, Lopez António, and Baptista Pedro Viana , Diagnostics, Volume 6, Issue 4, p.43, (2016) AbstractWebsite

The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted developments in the exploration of biomolecular interactions with AuNP-containing systems, in particular for biomedical applications in diagnostics. These systems show great promise in improving sensitivity, ease of operation and portability. Despite this endeavor, most platforms have yet to reach maturity and make their way into clinics or points of care (POC). Here, we present an overview of emerging and available molecular diagnostics using AuNPs for biomedical sensing that are currently being translated to the clinical setting.

Liposomes as Delivery System of a Sn(IV) Complex for Cancer Therapy, Corvo, Luísa M., Mendo Ana Soraia, Figueiredo Sara, Gaspar Rogério, Larguinho Miguel, da Silva Fátima Guedes M. C., Baptista Pedro Viana, and Fernandes Alexandra R. , Pharmaceutical Research, (2016)
Synthesis, Characterization, Thermal Properties and Antiproliferative Potential of Copper(II) 4′-phenyl-terpyridine Compounds, Ma, Zhen, Zhang Bian, da Silva Fátima Guedes M. C., Silva Joana, Mendo Ana Soraia, Baptista Pedro Viana, and Fernandes Alexandra R. , Dalton Transactions, Volume 45, Issue 12, (2016)
Colorimetric assessment of BCR-ABL1 transcripts in clinical samples via gold nanoprobes, Vinhas, Raquel, Correia Claudia, Ribeiro Patricia, Lourenco Alexandra, de Sousa Aida Botelho, Fernandes Alexandra R., and Baptista Pedro V. , Analytical and Bioanalytical Chemistry, Volume 408, Number 19, p.5277-5284, (2016) Abstract

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Gold Nanoparticles for Diagnostics: Advances towards Points of Care, Cordeiro, Milton, Carlos Fabio Ferreira, Pedrosa Pedro, Lopez Antonio, and Baptista Pedro Viana , Diagnostics, Volume 6, Number 4, (2016) Abstract

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Heteroleptic mononuclear compounds of ruthenium(II): synthesis, structural analyses, in vivo antitumor activity and in vivo toxicity on zebrafish embryost, Lenis-Rojas, O. A., Fernandes A. R., Roma-Rodrigues C., Baptista P. V., Marques F., Perez-Fernandez D., Guerra-Varela J., Sanchez L., Vazquez-Garcia D., Lopez Torres M., Fernandez A., and Fernandez J. J. , Dalton Transactions, Volume 45, Number 47, p.19127-19140, (2016) Abstract

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In vitro and in vivo biological characterization of the anti-proliferative potential of a cyclic trinuclear organotin(IV) complex, Martins, Marta, Baptista Pedro V., Mendo Ana Soraia, Correia Claudia, Videira Paula, Rodrigues Antonio S., Muthukumaran J., Santos-Silva Teresa, Silva Ana, Guedes da Silva Fatima M. C., Gigante Joana, Duarte Antonio, Gajewska Malgorzata, and Fernandes Alexandra R. , Molecular Biosystems, Volume 12, Number 3, p.1015-1023, (2016) Abstract

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Infection of human keratinocytes by Streptococcus dysgalactiae subspecies dysgalactiae isolated from milk of the bovine udder, Roma-Rodrigues, Catarina, Alves-Barroco Cynthia, Raposo Luis R., Costa Mafalda N., Fortunato Elvira, Baptista Pedro Viana, Fernandes Alexandra R., and Santos-Sanches Ilda , Microbes and Infection, Volume 18, Number 4, p.290-293, (2016) Abstract

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Liposomes as Delivery System of a Sn(IV) Complex for Cancer Therapy, Luisa Corvo, M., Mendo Ana Soraia, Figueiredo Sara, Gaspar Rogerio, Larguinho Miguel, Guedes da Silva Fatima M. C., Baptista Pedro Viana, and Fernandes Alexandra R. , Pharmaceutical Research, Volume 33, Number 6, p.1351-1358, (2016) Abstract

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Non-small cell lung cancer biomarkers and targeted therapy - two faces of the same coin fostered by nanotechnology, Mendes, Rita, Carreira Barbara, Baptista Pedro V., and Fernandes Alexandra R. , Expert Review of Precision Medicine and Drug Development, Volume 1, Number 2, p.155-168, (2016) Abstract

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A novel mutation in CEBPA gene in a patient with acute myeloid leukemia, Vinhas, Raquel, Tolmatcheva Anna, Canto Rafaela, Ribeiro Patricia, Lourenco Alexandra, de Sousa Aida Botelho, Baptista Pedro V., and Fernandes Alexandra R. , Leukemia & Lymphoma, Volume 57, Number 3, p.711-713, (2016) Abstract

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Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo, Roma-Rodrigues, Catarina, Heuer-Jungemann Amelie, Fernandes Alexandra R., Kanaras Antonios G., and Baptista Pedro V. , International Journal of Nanomedicine, Volume 11, p.2633-2639, (2016) Abstract

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Precision nanomedicine in cancer: how far are we from personalization?, Baptista, Pedro Viana , Expert Review of Precision Medicine and Drug Development, Volume 1, Number 3, p.227-228, (2016) Abstract

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2015
15 years on siRNA delivery: Beyond the State-of-the-Art on inorganic nanoparticles for RNAi therapeutics, Conde, João, Ambrosone Alfredo, Hernandez Yulán, Tian Furong, McCully Mark, Berry Catherine C., Baptista Pedro V., and T Claudia , NANO TODAY, Volume In Press, (2015) Abstract

RNAi has always captivated scientists due to its tremendous power to modulate the phenotype of living organisms. This natural and powerful biological mechanism can now be harnessed to down-regulate specific gene expression in diseased cells; opening up endless opportunities. Since most of the conventional siRNA delivery methods are limited by a narrow therapeutic index and significant side and off-target effects, we are now in the dawn of a new age in gene therapy driven by nanotechnology vehicles for RNAi therapeutics. Here, we outlook the "do's and dont's" of the inorganic RNAi nanomaterials developed in the last 15 years and the different strategies employed are compared and scrutinized, offering important suggestions for the next 15.

Characterization of antiproliferative potential and biological targets of a copper compound containing 4'-phenyl terpyridine, Mendo, Ana Soraia, Figueiredo Sara, Roma-Rodrigues Catarina, Videira Paula A., Ma Zhen, Diniz Mário, Larguinho Miguel, Costa Pedro, Lima João C., Pombeiro Armando J. L., Baptista Pedro V., and Fernandes Alexandra R. , JBIC Journal of Biological Inorganic Chemistry, Volume 2, Issue 6, p.935-948, (2015) AbstractWebsite

Several copper complexes have been assessed as anti-tumor agents against cancer cells. In this work, a copper compound [Cu(H2O){OS(CH3)2}L](NO3)2 incorporating the ligand 4'-phenyl-terpyridine antiproliferative activity against human colorectal, hepatocellular carcinomas and breast adenocarcinoma cell lines was determined, demonstrating high cytotoxicity. The compound is able to induce apoptosis and a slight delay in cancer cell cycle progression, probably by its interaction with DNA and induction of double-strand pDNA cleavage, which is enhanced by oxidative mechanisms. Moreover, proteomic studies indicate that the compound induces alterations in proteins involved in cytoskeleton maintenance, cell cycle progression and apoptosis, corroborating its antiproliferative potential.

Gold and silver nanoparticles for diagnostics of infection, Pedrosa, Pedro, and Baptista Pedro Viana , Nanotechnology in Diagnosis, Treatment and Prophylaxis of Infectious Diseases, p.1-18, (2015) Abstract

Nanotechnology in Diagnosis, Treatment and Prophylaxis of Infectious Diseases delivers comprehensive coverage of the application of nanotechnology to pressing problems in infectious disease.
This text equips readers with cutting-edge knowledge of promising developments and future prospects in nanotechnology, paying special attention to microbes that are now resistant to conventional antibiotics, a concerning problem in modern medicine.
Readers will find a thorough discussion of this new approach to infectious disease treatment, including the reasons nanotechnology presents a promising avenue for the diagnosis, treatment, and prophylaxis of infectious diseases.

Gold nanoparticle-based theranostics: disease diagnostics and treatment using a single nanomaterial, Vinhas, Raquel, Cordeiro Milton, Carlos Fábio, Mendo Soraia, Fernandes Alexandra, Figueiredo Sara, and Baptista Pedro , Journal of Nanobiosensors in Disease Diagnosis, Issue 4, p.11-23, (2015) AbstractWebsite

Nanotheranostics takes advantage of nanotechnology-based systems in order to diagnose and treat a specific disease. This approach is particularly relevant for personalized medicine, allowing the detection of a disease at an early stage, to direct a suitable therapy toward the target tissue based on the molecular profile of the altered phenotype, subsequently facilitating disease monitoring and following treatment. A tailored strategy also enables to reduce the off-target effects associated with universal treatments and improve the safety profile of a given treatment. The unique optical properties of gold nanoparticles, their ease of surface modification, and high surface-to-volume ratio have made them central players in this area. By combining imaging, targeting, and therapeutic agents in a single vehicle, these nanoconjugates are (ought to be) an important tool in the clinics. In this review, the multifunctionality of gold nanoparticles as theranostics agents will be highlighted, as well as the requirements before the translation of these nanoplatforms into routine clinical practice.