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Welcome to the SeaTox Lab website

Our mission

The SeaTox Lab aims at understanding how marine fauna respond and have evolved to cope with chemical aggressors, whether anthropogenic (pollutants) or biogenic (toxins). This includes investigating how marine animals acquired the ability to produce toxins as "warfare" to avoid parasites, predation or to become predators thamselves. Our mission is to integrate the toxicologist's perspective within Marine Environmental Research and Marine biotechnology.

Expertise

The SeaTox Lab is a multidisciplinary group, as toxicology and biotechnology involve the integration of multiple domains of expertise and methodologies. We highlight the following:

Ecology, biology and evolution of aquatic organisms

•Toxicopathological biomarkers: from transcriptome and proteome to pathological traits

Screening for novel toxicological pathways

Substance testing: evaluating hazards and risks of pollutants and novel drugs, with emphasis on genotoxicants, mutagens and carcinogens

Animal histology, cytology and histopathology: from aquatic invertebrates to mammals

Omics, multi-omics and bioinformatics in toxicity-testing and drug discovery, with emphasis on proteomics and transcriptomics.

The team @SeaTox

The single cell gel electrophoresis assay (SCGE), or simply the 'Comet' assay, quantitates damaged DNA in isolated cells follawing embedding in agarose, lysis and electrophoresis in extremely alkaline conditions. This protocol is one of the most widely used for genotoxicologists to study the effects of noxious compounds onto DNA. The image shows the 'tail' of damaged DNA (containing fragments and relaxed loops) migrating from what is left of the nucleus of a cell (now termed 'nucleoid'), after staining with a DNA-intercalating fluorochrome and magnified 400 times. More DNA in the 'tail' meains more damage to DNA, which can be quatified through image analysis. 
Histology, immunohistochemistry, histopathology and related domains are one of our most important expertise. We work with a wide range of animals (vertebrates and invertebrates), for a broad scope of research projects: from substance testing to standard pathology. We are always open to new collaborations and challenges. Clockwise: digestive gland of a marine mussel, stained with one of our very-own cocktails of dyes; section across the retina of the zebrafish (fluorescence microscopy); semi-thin resin section across the ventral area of an annelid, showing the two nerve cords. Check "The Handbook of Histopathological Practices in Aquatic Environments: Guide to Histology for Environmental Toxicology".

Ongoing and recent research projects

Project MARVEN (leading lab.)

Project WormALL (leading lab.)

Project GreenTech (leading lab.) Just closed!

Project MBStox (participant)

 Project "3-Qs for Quality" (participant) Just closed!

For more information, see our funded research page, here

[Site last updated: 04-07-2020]

The SeaTox Lab team early January 2020. Up, left to right: A.P. Rodrigo, C. Gonçalves, C. Martins, P.M. Costa., M. D'Ambrosio, C. Madeira, M. Grou, L. Santos. Bottom, left to right: I. Ramos, R. Pereira, R. Estevens, R. Bastos.