Publications

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Vinhas, Raquel, Claudia Correia, Patricia Ribeiro, Alexandra Lourenco, Aida Botelho de Sousa, Alexandra R. Fernandes, and Pedro V. Baptista. "Colorimetric assessment of BCR-ABL1 transcripts in clinical samples via gold nanoprobes." Analytical and Bioanalytical Chemistry 408 (2016): 5277-5284. Abstract

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Baptista, P., G. Doria, D. Henriques, E. Pereira, and R. Franco. "Colorimetric detection of eukaryotic gene expression with DNA-derivatized gold nanoparticles." Journal of Biotechnology 119 (2005): 111-117. Abstract

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Pedrosa, Pedro, Rita Mendes, Rita Cabral, Luisa M. D. R. S. Martins, Pedro V. Baptista, and Alexandra R. Fernandes. "Combination of chemotherapy and Au-nanoparticle photothermy in the visible light to tackle doxorubicin resistance in cancer cells." Scientific Reports 8 (2018). Abstract

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FLOMEN, RH, R. VATCHEVA, PA GORMAN, and al.et. "Construction and analysis of a sequence-ready map in 4q25: Rieger syndrome can be caused by haploinsufficiency of RIEG, but also by chromosome breaks approximate to 90 kb upstream of this gene." GENOMICS 47 (1998): 409-413. Abstract

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FLOMEN, R., PB BAPTISTA, CH JAMES, and al.et. "Construction of a YAC/PAC physical map of a gene rich region in 1p13.3." EUROPEAN JOURNAL OF HUMAN GENETICS 6 (1998): 168. Abstract

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Machado, Diana, Isabel Couto, João Perdigão, Liliana Rodrigues, Isabel Portugal, Pedro Baptista, Bruno Veigas, Leonard Amaral, and Miguel Viveiros. "Contribution of efflux to the emergence of isoniazid and multidrug resistance in Mycobacterium tuberculosis." PLoS ONE 7 (2012): e34538.
Baptista, Pedro Viana. "Could gold nanoprobes be an important tool in cancer diagnostics?" Expert Review of Molecular Diagnostics 12 (2012): 541-543. Abstract

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Roma-Rodrigues, C., A. R. Fernandes, and P. V. Baptista. "Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles." Int J Nanomedicine 14 (2019): 6843-6854. AbstractWebsite

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Cordeiro, M., L. Giestas, J. C. Lima, and Pedro Viana Baptista. "Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis." J Biotechnol 168 (2013): 90-94. AbstractWebsite

We previously reported a strategy that combines Förster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.
Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair.

Giestas, L., J. C. Lima, and P. V. Baptista. "Coupling single base extension to a spectral codification tool for increased throughput screening." J Biotechnol 154 (2011): 199-204. AbstractWebsite

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Vinhas, Raquel, Milton Cordeiro, Pedro Pedrosa, Alexandra R. Fernandes, and Pedro V. Baptista. "Current trends in molecular diagnostics of chronic myeloid leukemia." Leukemia & Lymphoma (2016): 1-14. AbstractWebsite

Nearly 1.5 million people worldwide suffer from chronic myeloid leukemia (CML), characterized by the genetic translocation t(9;22)(q34;q11.2), involving the fusion of the Abelson oncogene (ABL1) with the breakpoint cluster region (BCR) gene. Early onset diagnosis coupled to current therapeutics allow for a treatment success rate of 90, which has focused research on the development of novel diagnostics approaches. In this review, we present a critical perspective on current strategies for CML diagnostics, comparing to gold standard methodologies and with an eye on the future trends on nanotheranostics.

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Conde, Joao, Alfredo Ambrosone, Vanesa Sanz, Yulan Hernandez, Valentina Marchesano, Furong Tian, Hannah Child, Catherine C. Berry, Ricardo M. Ibarra, Pedro V. Baptista, Claudia Tortiglione, and Jesus M. de la Fuente. "Design of Multifunctional Gold Nanoparticles for In Vitro and In Vivo Gene Silencing." Acs Nano 6 (2012): 8316-8324. Abstract

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Conde, J., A. Ambrosone, Y. Hernandez, V. Marchesano, F. Tian, M. Ricardo Ibarra, P. V. Baptista, C. Tortiglione, and J. M. de la Fuente. "Designing gold nanoparticles for in vivo gene silencing as a new therapeutic tool." Human Gene Therapy 24 (2013): A24. Abstract

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Larguinho, Miguel, Hugo M. Santos, Gonçalo Doria, H. Scholz, Pedro V. Baptista, and José L. Capelo. "Development of a fast and efficient ultrasonic-based strategy for DNA fragmentation." J. Talanta 81 (2010): 881-886.
Coelho, Beatriz, Bruno Veigas, Elvira Fortunato, Rodrigo Martins, Hugo Aguas, Rui Igreja, and Pedro V. Baptista. "Digital Microfluidics for Nucleic Acid Amplification." Sensors 17 (2017). Abstract

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Coelho, Beatriz Jorge, Bruno Veigas, Hugo Aguas, Elvira Fortunato, Rodrigo Martins, Pedro Viana Baptista, and Rui Igreja. "A Digital Microfluidics Platform for Loop-Mediated Isothermal Amplification Detection." Sensors 17 (2017). Abstract

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Baptista, P., G. Doria, A. Matias, L. Osorio, E. Pereira, and F. Ricardo. Direct detection of mutations/SNPs using DNA-derivatised gold nanoparticles In Congress of Clinical Chemistry and Laboratory Medicine. Jena, Germany, 2005.
Larguinho, Miguel, Sofia Santos, Joao Almeida, and Pedro V. Baptista. "DNA adduct identification using gold-aptamer nanoprobes." Iet Nanobiotechnology 9 (2015): 95-101. Abstract

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Vinhas, Raquel, Alexandra Lourenco, Susana Santos, Patricia Ribeiro, Madalena Silva, Aida Botelho de Sousa, Pedro V. Baptista, and Alexandra R. Fernandes. "A double Philadelphia chromosome-positive chronic myeloid leukemia patient, co-expressing P210(BCR-ABL1) and P195(BCR-ABL1) isoforms." Haematologica 103 (2018): E549-E552. Abstract

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Reimão-Pinto, M. M., A. Cordeiro, C. Almeida, A. V. Pinheiro, A. Moro, J. C. Lima, and P. V. Baptista. "Dual-color control of nucleotide polymerization sensed by a fluorescence actuator." Photochem Photobiol Sci 13 (2014): 751-6. AbstractWebsite

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Conde, Joao, Furong Tian, Jesus M. de la Fuente, and Pedro V. Baptista. "Editorial: Cancer Nanotheranostics: What Have We Learned So Far?" Frontiers in Chemistry 71 (2016).Website
Sanz, Vanesa, João Conde, Yulán Hernández, Pedro V. Baptista, M. R. Ibarra, and Jesús M. de la Fuente. "Effect of PEG biofunctional spacers and TAT peptide on dsRNA loading on gold nanoparticles." J. Nanoparticles Res. 14 (2012): 917.
dos Santos, Margarida Moreira, Margarida João Queiroz, and Pedro V. Baptista. "Enhancement of antibiotic effect via gold:silver alloy nanoparticles." J. Nanopar. Res. 14 (2012): 859.
Jesus, Ana R., Mario R. C. Soromenho, Luis R. Raposo, Jose M. S. S. Esperanca, Pedro V. Baptista, Alexandra R. Fernandes, and Patricia M. Reis. "Enhancement of water solubility of poorly water-soluble drugs by new biocompatible N-acetyl amino acid N-alkyl cholinium-based ionic liquids." European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (2019). Abstract

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Ribeiro, A. P. C., S. Anbu, E. C. B. A. Alegria, A. R. Fernandes, P. V. Baptista, R. Mendes, A. S. Matias, M. Mendes, M. F. C. Guedes da Silva, and A. J. L. Pombeiro. "Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles." Biomedicine & Pharmacotherapy 101 (2018): 137-144. AbstractWebsite

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