Publications

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2014
Larguinho, Miguel, Daniela Correia, Mario S. Diniz, and Pedro V. Baptista. "Evidence of one-way flow bioaccumulation of gold nanoparticles across two trophic levels." Journal of Nanoparticle Research 16 (2014). Abstract

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Luis, Daniel V., Joana Silva, Ana Isabel Tomaz, Rodrigo F. M. de Almeida, Miguel Larguinho, Pedro V. Baptista, Luisa M. D. R. S. Martins, Telma F. S. Silva, Pedro M. Borralho, Cecilia M. P. Rodrigues, Antonio S. Rodrigues, Armando J. L. Pombeiro, and Alexandra R. Fernandes. "Insights into the mechanisms underlying the antiproliferative potential of a Co(II) coordination compound bearing 1,10-phenanthroline-5,6-dione: DNA and protein interaction studies." Journal of Biological Inorganic Chemistry 19 (2014): 787-803. Abstract

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Larguinho, Miguel, Ana Cordeiro, Mario S. Diniz, Pedro M. Costa, and Pedro V. Baptista. "Metabolic and histopathological alterations in the marine bivalve Mytilus galloprovincialis induced by chronic exposure to acrylamide." Environmental Research 135 (2014): 55-62. Abstract

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Quaresma, Pedro, Ines Osorio, Goncalo Doria, Patricia A. Carvalho, Andre Pereira, Judith Langer, Joao Pedro Araujo, Isabel Pastoriza-Santos, Luis M. Liz-Marzan, Ricardo Franco, Pedro V. Baptista, and Eulalia Pereira. "Star-shaped magnetite@gold nanoparticles for protein magnetic separation and SERS detection." Rsc Advances 4 (2014): 3659-3667. AbstractWebsite

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2013
Bernacka-Wojcik, Iwona, Paulo Lopes, Ana Catarina Vaz, Bruno Veigas, Pawel Jerzy Wojcik, Pedro Simões, David Barata, Elvira Fortunato, Pedro V. Baptista, Hugo Águas, and Rodrigo Martins. "Bio-microfluidic platform for gold nanoprobe based DNA detection—application to Mycobacterium tuberculosis." Biosens Bioelectron 48 (2013): 87-93. AbstractWebsite

We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 µl on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.

Cordeiro, M., L. Giestas, J. C. Lima, and Pedro Viana Baptista. "Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis." J Biotechnol 168 (2013): 90-94. AbstractWebsite

We previously reported a strategy that combines Förster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.
Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair.

Larguinho, Miguel, José L. Capelo, and Pedro V. Baptista. "Fast Nucleotide identification using gold nanoparticle-based surface assisted laser desorption/ionisation." Talanta 105 (2013): 417-421.
Larguinho, M., J. L. Capelo, and P. V. Baptista. "Fast nucleotide identification through fingerprinting using gold nanoparticle-based surface-assisted laser desorption/ionisation." Talanta 105 (2013): 417-21. AbstractWebsite

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2012
Larguinho, Miguel, and Pedro V. Baptista. "Gold and silver nanoparticles for clinical diagnostics - from genomics to proteomics." J. Proteomics 75 (2012): 2811-2823.
Conde, João, João Rosa, João C. Lima, and Pedro V. Baptista. "Nanophotonics for Molecular Diagnostics and Therapy Applications." Int. J. Photoenergy 619530 (2012).
Rosa, J., J. Conde, J. M. de la Fuente, J. C. Lima, and P. V. Baptista. "Gold-nanobeacons for real-time monitoring of RNA synthesis." Biosens Bioelectron 36 (2012): 161-7. AbstractWebsite

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Conde, J., J. Rosa, J. C. Lima, and P. V. Baptista. "Nanophotonics for Molecular Diagnostics and Therapy Applications." International Journal of Photoenergy (2012). Abstract

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2011
Giestas, Letícia, João Carlos Lima, and Pedro V. Baptista. " Coupling single base extension to a spectral codification tool for increased throughput screening." J. Biotechnol. 154 (2011): 199-204.
Rosa, J. P., J. C. Lima, and P. V. Baptista. "Experimental photophysical characterization of fluorophores on the vicinity of gold nanoparticles - deciphering disputes related to kr determination." Nanotechnology 22 (2011): 415202.
Giestas, L., J. C. Lima, and P. V. Baptista. "Coupling single base extension to a spectral codification tool for increased throughput screening." J Biotechnol 154 (2011): 199-204. AbstractWebsite

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Rosa, J. P., J. C. Lima, and P. V. Baptista. "Experimental photophysical characterization of fluorophores in the vicinity of gold nanoparticles." Nanotechnology 22 (2011). Abstract

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2010
Doria, G., J. T. Dias, M. Larguinho, E. Pereira, R. Franco, and P. Baptista. AuAg-alloy-nanoprobes for Specific Nucleic Acid Detection In NSTI-Nanotech 2010. Anaheim, CA, 2010.
Larguinho, Miguel, Hugo M. Santos, Gonçalo Doria, H. Scholz, Pedro V. Baptista, and José L. Capelo. "Development of a fast and efficient ultrasonic-based strategy for DNA fragmentation." J. Talanta 81 (2010): 881-886.
Pinheiro, André, Jorge A. Parola, Pedro Baptista, and João Carlos Lima. "pH effect on the photochemistry of 4-methylcoumarin phosphate esters: Caged-Phosphate Case Study." 2010 114 (2010): 12795-12803.
Doria, G., M. Larguinho, J. T. Dias, E. Pereira, R. Franco, and P. V. Baptista. "Gold-silver-alloy nanoprobes for one-pot multiplex DNA detection." Nanotechnology 21 (2010): 255101. AbstractWebsite

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Pinheiro, A. V., J. Conde, A. J. Parola, J. C. Lima, and P. V. Baptista. "Use of cyclodextrins as scavengers of inhibitory photo-products in light controlled in vitro synthesis of RNA." Journal of Photochemistry and Photobiology a-Chemistry 213 (2010): 147-151. Abstract

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2009
Giestas, Letícia, Vesselin Petrov, Pedro V. Baptista, and João Carlos Lima. "General FRET-based coding for application in multiplexing methods." Photochem. Photobiol. Sci. 8 (2009): 1130-1138.
2008
Pinheiro, André Vidal, Pedro Baptista, and João Carlos Lima. "Light activation of transcription: photocaging of nucleotides for control over RNA polymerization." Nucleic Acids Res. 36 (2008): 90.
Giestas, Letícia, Guilherme N. M. Ferreira, Pedro V. Baptista, and João Carlos Lima. "Multiplexed spectral coding for simultaneous detection of DNA hybridization reactions based on FRET." Sens. Actuator B-Chem. 134 (2008): 146-157.
Pinheiro, A. V., P. Baptista, and J. C. Lima. "Light activation of transcription: photocaging of nucleotides for control over RNA polymerization." Nucleic Acids Res 36 (2008): e90. AbstractWebsite

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