Roque, ACA, Lowe CR.
2007.
Affinity chromatography: History, Perspectives, Limitations and Prospects. Affinity Chromatography: Methods and Protocols. (
M. Zachariou, Ed.).:1-23., U.S.A.: Humana Press Inc.
AbstractBiomolecule separation and purification has until very recently steadfastly remained one of the more empirical aspects of modern biotechnology. Affinity chromatography, one of several types of adsorption chromatography, is particularly suited for the efficient isolation of biomolecules. This technique relies on the adsorbent bed material that has biological affinity for the substance to be isolated. This review is intended to place affinity chromatography in historical perspective and describe the current status, limitations and future prospects for the technique in modern biotechnology.
Pina, AS, Batalha IL, Roque ACA.
2014.
Affinity Tags in Protein Purification and Peptide Enrichment: An Overview. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods. (
Labrou, Nikolaos, Ed.).:147-168.: Springer
AbstractThe reversible interaction between an affinity ligand and a complementary receptor has been widely explored in purification systems for several biomolecules. The development of tailored affinity ligands highly specific towards particular target biomolecules is one of the options in affinity purification systems. However, both genetic and chemical modifications on proteins and peptides widen the application of affinity ligand-tag receptor pairs towards universal capture and purification strategies. In particular, this chapter will focus on two case studies highly relevant for biotechnology and biomedical areas, namely, the affinity tags and receptors employed on the production of recombinant fusion proteins and the chemical modification of phosphate groups on proteins and peptides and the subsequent specific capture and enrichment, a mandatory step before further proteomic analysis.