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Electrochemical studies of the hexaheme nitrite reductase from Desulfovibrio desulfuricans ATCC 27774, Moreno, C., Costa C., Moura I., Legall J., Liu M. Y., Payne W. J., Van Dijk C., and Moura J. J. , Eur J Biochem, Feb 15, Volume 212, Number 1, p.79-86, (1993) AbstractWebsite

The electron-transfer kinetics between three different mediators and the hexahemic enzyme nitrite reductase isolated from Desulfovibrio desulfuricans (ATCC 27774) were investigated by cyclic voltammetry and by chronoamperometry. The mediators, methyl viologen, Desulfovibrio vulgaris (Hildenborough) cytochrome c3 and D. desulfuricans (ATCC 27774) cytochrome c3 differ in structure, redox potential and charge. The reduced form of each mediator exchanged electrons with nitrite reductase. Second-order rate constants, k, were calculated on the basis of the theory for a simple catalytic mechanism and the results, obtained by cyclic voltammetry, were compared with those obtained by chronoamperometry. Values for k are in the range 10(6)-10(8) M-1 s-1 and increase in the direction D. desulfuricans cytochrome c3-->D. vulgaris cytochrome c3-->methyl viologen. An explanation is advanced on the basis of electrostatic interactions and relative orientation between the partners involved. Chronoamperometry (computer controlled) offers advantages over cyclic voltammetry in the determination of homogeneous rate constants (faster, more accurate and better reproducibility). Direct, unmediated electrochemical responses of the hexaheme nitrite reductase were also reported.

Redox properties of Desulfovibrio gigas [Fe3S4] and [Fe4S4] ferredoxins and heterometal cubane-type clusters formed within the [Fe3S4] core. Square wave voltammetric studies, Moreno, C., Macedo A. L., Moura I., Legall J., and Moura J. J. , J Inorg Biochem, Feb 15, Volume 53, Number 3, p.219-34, (1994) AbstractWebsite

The same polypeptide chain (58 amino acids, 6 cysteines) is used to build up two ferredoxins in Desulfovibrio gigas a sulfate reducing organism. Ferredoxin II (FdII) contains a single [Fe3S4] core and ferredoxin I (FdI) mainly a [Fe4S4] core. The [Fe3S4] core can readily be interconverted into a [Fe4S4] complex (J.J.G. Moura, I. Moura, T.A. Kent, J.D. Lipscomb, B.H. Huynh, J. LeGall, A.V. Xavier, and E. Munck, J. Biol. Chem. 257, 6259 (1982)). This interconversion process suggested that the [Fe3S4] core could be used as a synthetic precursor for the formation of heterometal clusters. Co, Zn, Cd, and Ni derivatives were produced (I. Moura, J.J.G. Moura, E. Munck, V. Papaephthymiou, and J. LeGall, J. Am. Chem. Soc. 108, 349 (1986), K. Sureurs, E. Munck, I. Moura, J.J.G. Moura, and J. LeGall, J. Am. Chem. Soc. 109, 3805 (1986), and A.L. Macedo, I. Moura, J.J.G. Moura, K. Surerus, and E. Munck, unpublished results). The redox properties of a series of heterometal clusters (MFe3S4] are assessed using direct electrochemistry (square wave voltammetry--SWV) promoted by Mg(II) at a glassy carbon electrode (derivatives: Cd (-495 mV), Fe (-420 mV), Ni (-360 mV), and Co (-245 mV) vs normal hydrogen electrode (NHE)). In parallel, the electrochemical behavior (cyclic voltammetry--CV, differential pulse voltammetry--DPV and SWV) of FdI and FdII were investigated as well as the cluster interconversion process. In addition to the +1/0 (3Fe cluster) and +2/+1 (4Fe cluster) redox transitions, a very negative redox step, at -690 mV, was detected for the 3Fe core, reminiscent of a postulated further 2e- reduction step, as proposed for D. africanus ferredoxin III by F.A. Armstrong, S.J. George, R. Cammack, E.C. Hatchikian, and A.J. Thomson, Biochem. J. 264, 265 (1989). The electrochemical redox potential values are compared with those determined by independent methods (namely by electron paramagnetic resonance (EPR) and visible spectroscopy).

Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491, Mota, C. S., Valette O., Gonzalez P. J., Brondino C. D., Moura J. J., Moura I., Dolla A., and Rivas M. G. , J Bacteriol, Jun, Volume 193, Number 12, p.2917-23, (2011) AbstractWebsite

Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 muM molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 muM tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed.

The mechanism of formate oxidation by metal-dependent formate dehydrogenases, Mota, C. S., Rivas M. G., Brondino C. D., Moura I., Moura J. J., Gonzalez P. J., and Cerqueira N. M. , J Biol Inorg Chem, Dec, Volume 16, Number 8, p.1255-68, (2011) AbstractWebsite

Metal-dependent formate dehydrogenases (Fdh) from prokaryotic organisms are members of the dimethyl sulfoxide reductase family of mononuclear molybdenum-containing and tungsten-containing enzymes. Fdhs catalyze the oxidation of the formate anion to carbon dioxide in a redox reaction that involves the transfer of two electrons from the substrate to the active site. The active site in the oxidized state comprises a hexacoordinated molybdenum or tungsten ion in a distorted trigonal prismatic geometry. Using this structural model, we calculated the catalytic mechanism of Fdh through density functional theory tools. The simulated mechanism was correlated with the experimental kinetic properties of three different Fdhs isolated from three different Desulfovibrio species. Our studies indicate that the C-H bond break is an event involved in the rate-limiting step of the catalytic cycle. The role in catalysis of conserved amino acid residues involved in metal coordination and near the metal active site is discussed on the basis of experimental and theoretical results.

Flavodoxin and rubredoxin from Desulphovibrio salexigens, Moura, I., Moura J. J., Bruschi M., and Legall J. , Biochim Biophys Acta, Jun 10, Volume 591, Number 1, p.1-8, (1980) AbstractWebsite

A flavodoxin and a rubredoxin have been isolated from the sulfate-reducing bacterium Desulphovibrio salexigens (strain British Guiana, NICB 8403). Their amino acid composition and spectral characteristics did not differ markedly from the homologous proteins presented in other Desulphovibrio spp. Flavodoxin was shown to be active in the electron transport of the sulfite reductase system.

Molybdenum and tungsten enzymes: from biology to chemistry and back, Moura, J. J. G., Bernhardt P. V., Maia L. B., and Gonzalez P. J. , J Biol Inorg Chem, Volume 20, p.181-182, (2015)
Characterization of the cytochrome system of a nitrogen-fixing strain of a sulfate-reducing bacterium: Desulfovibrio desulfuricans strain Berre-Eau, Moura, I., Fauque G., Legall J., Xavier A. V., and Moura J. J. , Eur J Biochem, Feb 2, Volume 162, Number 3, p.547-54, (1987) AbstractWebsite

Two c-type cytochromes were purified and characterized by electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) spectroscopic techniques, from the sulfate-reducer nitrogen-fixing organism, Desulfovibrio desulfuricans strain Berre-Eau (NCIB 8387). The purification procedures included several chromatographic steps on alumina, carboxymethylcellulose and gel filtration. A tetrahaem and a monohaem cytochrome were identified. The multihaem cytochrome has visible, EPR and NMR spectra with general properties similar to other low-potential bis-histidinyl axially bound haem proteins, belonging to the class of tetrahaem cytochrome c3 isolated from other Desulfovibrio species. The monohaem cytochrome c553 is ascorbate-reducible and its EPR and NMR data are characteristic of a cytochrome with methionine-histidine ligation. Their properties are compared with other homologous proteins isolated from sulfate-reducing bacteria.

Ferredoxin from Methanosarcina barkeri: evidence for the presence of a three-iron center, Moura, I., Moura J. J., Huynh B. H., Santos H., Legall J., and Xavier A. V. , Eur J Biochem, Aug, Volume 126, Number 1, p.95-8, (1982) AbstractWebsite

Methanosarcina barkeri ferredoxin was purified and characterized by electron paramagnetic resonance (EPR) and Mossbauer spectroscopy. The purification procedure included chromatographic steps on DEAE-cellulose and gel filtration. The isolated protein is unstable under aerobic conditions. The ferredoxin exhibits charge transfer bands at 283 nm and 405 nm with an absorption ratio A405/A283 = 0.73. Its molecular weight has been estimated to be 20000-22000 by gel filtration chromatography. The native ferredoxin exhibits an intense EPR signal at g = 2.02 and only a very weak g = 1.94 signal develops upon reduction with dithionite. The Mossbauer spectra of the reduced protein are characteristic of a [3Fe-3S] center. The combined EPR and Mossbauer studies show that M. barkeri ferredoxin contains only [3Fe-3S] clusters, similar to Azotobacter vinelandii Fd[Emptage, M.H., Kent, T.A., Huynh, B.H., Rawlings, J., Orme-Johnson, W.H. & Munck, M. (1980) J. Biol. Chem. 255, 1793-1796], Desulfovibrio gigas FdII [Huynh, B.H., Moura, J.J.G., Moura, I., Kent, T.A., LeGall, J., Xavier, A.V. & Munck, E. (1980) J. Biol. Chem. 255, 3242-3244] and mitochondrial beef heart aconitase [Kent, T.A., Dreyer, J.-L., Kennedy, M.C., Huynh, B.H., Emptage, M.H., Beinert, H. & Munck, E. (1982) Proc. Natl Acad. Sci. USA, 79, 1096-1100].

Mo and W bis-MGD enzymes: nitrate reductases and formate dehydrogenases, Moura, J. J., Brondino C. D., Trincao J., and Romao M. J. , J Biol Inorg Chem, Oct, Volume 9, Number 7, p.791-9, (2004) AbstractWebsite

Molybdenum and tungsten are second- and third-row transition elements, respectively, which are found in a mononuclear form in the active site of a diverse group of enzymes that generally catalyze oxygen atom transfer reactions. Mononuclear Mo-containing enzymes have been classified into three families: xanthine oxidase, DMSO reductase, and sulfite oxidase. The proteins of the DMSO reductase family present the widest diversity of properties among its members and our knowledge about this family was greatly broadened by the study of the enzymes nitrate reductase and formate dehydrogenase, obtained from different sources. We discuss in this review the information of the better characterized examples of these two types of Mo enzymes and W enzymes closely related to the members of the DMSO reductase family. We briefly summarize, also, the few cases reported so far for enzymes that can function either with Mo or W at their active site.

[15] Characterization of three proteins containing multiple iron sites: Rubrerythrin, desulfoferrodoxin, and a protein containing a six-iron cluster, Moura, Isabel, Tavares Pedro, and Ravi Natarajan , Methods in Enzymology, Volume Volume 243, p.216-240, (1994) Abstract
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Assignment of individual heme EPR signals of Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3. A redox equilibria study, Moura, I., Teixeira M., Huynh B. H., Legall J., and Moura J. J. , Eur J Biochem, Sep 15, Volume 176, Number 2, p.365-9, (1988) AbstractWebsite

An EPR redox titration was performed on the tetraheme cytochrome c3 isolated from Desulfovibrio baculatus (strain 9974), a sulfate-reducer. Using spectral differences at different poised redox states of the protein, it was possible to individualize the EPR g-values of each of the four hemes and also to determine the mid-point redox potentials of each individual heme: heme 4 (-70 mV) at gmax = 2.93, gmed = 2.26 and gmin = 1.51; heme 3 (-280 mV) at gmax = 3.41; heme 2 (-300 mV) at gmax = 3.05, gmed = 2.24 and gmin = 1.34; and heme 1 (-355 mV) at gmx = 3.18. A previously described multi-redox equilibria model used for the interpretation of NMR data of D. gigas cytochrome c3 [Santos, H., Moura, J.J.G., Moura, I., LeGall, J. & Xavier, A. V. (1984) Eur. J. Biochem. 141, 283-296] is discussed in terms of the EPR results.

Isolation of P590 from Methanosarcina barkeri: evidence for the presence of sulfite reductase activity, Moura, J. J., Moura I., Santos H., Xavier A. V., Scandellari M., and Legall J. , Biochem Biophys Res Commun, Oct 15, Volume 108, Number 3, p.1002-9, (1982) AbstractWebsite
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Enzymatic activity mastered by altering metal coordination spheres, Moura, I., Pauleta S. R., and Moura J. J. , J Biol Inorg Chem, Nov, Volume 13, Number 8, p.1185-95, (2008) AbstractWebsite

Metalloenzymes control enzymatic activity by changing the characteristics of the metal centers where catalysis takes place. The conversion between inactive and active states can be tuned by altering the coordination number of the metal site, and in some cases by an associated conformational change. These processes will be illustrated using heme proteins (cytochrome c nitrite reductase, cytochrome c peroxidase and cytochrome cd1 nitrite reductase), non-heme proteins (superoxide reductase and [NiFe]-hydrogenase), and copper proteins (nitrite and nitrous oxide reductases) as examples. These examples catalyze electron transfer reactions that include atom transfer, abstraction and insertion.

Isolation and characterization of desulforedoxin, a new type of non-heme iron protein from Desulfovibrio gigas, Moura, I., Bruschi M., Legall J., Moura J. J., and Xavier A. V. , Biochem Biophys Res Commun, Apr 25, Volume 75, Number 4, p.1037-44, (1977) AbstractWebsite
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The Role Of Nickel And Iron Sulfur Centers In The Bioproduction Of Hydrogen, Moura, J. J. G., Teixeira M., and Moura I. , Pure and Applied Chemistry, May, Volume 61, Number 5, p.915-921, (1989) AbstractWebsite
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NMR studies of a dihaem cytochrome from Pseudomonas perfectomarinus (ATCC 14405), Moura, I., Liu M. C., Legall J., Peck, H. D. Jr., Payne W. J., Xavier A. V., and Moura J. J. , Eur J Biochem, Jun 1, Volume 141, Number 2, p.297-303, (1984) AbstractWebsite

Pseudomonas perfectomarinus (ATCC 14405) dihaem cytochrome c552 was studied by 300-MHz proton magnetic resonance. Some of the haem resonances were assigned in the fully reduced and fully oxidized states. No evidence was found for methionine haem axial coordination. The oxidation-reduction equilibrium was studied in detail. Due to the large difference in mid-point redox potential between the two haems (+174 mV, for haem II and -180 mV for haem I) an intermediate oxidation state could be obtained containing reduced haem I and oxidized haem II. In this way the total paramagnetic shift at different oxidation levels could be decomposed in the intrinsic and extrinsic contributions. It was found that the two haems interact. The rate of electron exchange is slow on the NMR time scale. The redox equilibria are discussed for four possible redox species in solution.

A molybdenum-containing (2Fe, 2S) protein from desulphovibrio gigas, a sulphate reducer, Moura, J. J. G., Xavier A. V., Bruschi M., Legall J., and Cabral J. M. P. , Journal of the Less Common Metals, Volume 54, Number 2, p.555-562, (1977) AbstractWebsite
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Spectroscopic studies of cobalt and nickel substituted rubredoxin and desulforedoxin, Moura, I., Teixeira M., Legall J., and Moura J. J. , J Inorg Biochem, Nov, Volume 44, Number 2, p.127-39, (1991) AbstractWebsite

The single iron site of rubredoxin was replaced by nickel and cobalt. The near-infrared/visible/UV spectra of these metal derivatives show ligand-field transitions and charge-transfer bands which closely resemble those of simple tetrathiolate complexes, indicating a tetrahedral arrangement of the sulfur cysteinyl ligands around the metal core. The 1H NMR spectra of the nickel and cobalt derivatives reveal extremely low-field contact shifted resonances of one proton intensity assigned to beta-CH2 and alpha-CH cysteinyl protons. Other well resolved resonances shifted out of the main protein spectral envelope are also observed and probably arise from contact plus pseudocontact shift mechanisms. Rubredoxins from different sulfate reducers were metal substituted and assignments of aliphatic protons are tentatively proposed, taking advantage of the amino acid sequence homologies. The present data is promising in terms of structural analysis of the coordination sphere of the metal core. It was also shown that replacement of the iron atom of desulforedoxin, a close analogue of rubredoxin, by cobalt and nickel was possible.

Nuclear-magnetic-resonance studies of Desulfuromonas acetoxidans cytochrome c551.5 (c7), Moura, José J. G., Moore Geoffrey R., Williams Robert J. P., Probst Irmelin, Legall Jean, and Xavier António V. , European Journal of Biochemistry, Volume 144, Number 3, p.433-440, (1984) AbstractWebsite

1H nuclear magnetic resonance (NMR) spectroscopy has been used to examine cytochrome c551.5 (c7) from the sulfur reducer, Desulfuromonas acetoxidans. This protein contains three hemes. Two stable oxidation states (the fully oxidized and the fully reduced) as well as intermediate oxidation states were studied. The axial ligands of the iron were found to be neutral histidines. The redox properties of cytochrome c7 were examined and good quantitative agreement found between the NMR results and previously reported redox potential measurements. The properties of cytochrome c7 are discussed together with those of the homologous tetraheme cytochromes c3 isolate from sulfate-reducing bacteria.

Structural control of the redox potentials and of the physiological activity by oligomerization of ferredoxin, Moura, J. J., Xavier A. V., Hatchikian E. C., and Legall J. , FEBS Lett, May 1, Volume 89, Number 1, p.177-9, (1978) AbstractWebsite
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A bird’s-eye view of denitrification in relation to the nitrogen cycle, Moura, I., Maia L. B., Pauleta S. R., and Moura J. J. G. , Metalloenzymes in Denitrification: Applications and Environmental Impacts, RSC Metallobiology Series No. 9 (ISBN: 978-1-78262-376-2)., Cambridge, p.1-10, (2017) n_cycle-rsc_book-denitrification-chap_1.pdf
Nitrate and nitrite utilization in sulfate-reducing bacteria, Moura, I., Bursakov S., Costa C., and Moura J. J. , Anaerobe, Oct, Volume 3, Number 5, p.279-90, (1997) AbstractWebsite
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Mossbauer And Electron-Paramagnetic-Res Studies Of Desulforedoxin From Desulfovibrio-Gigas, Moura, I., Huynh B. H., Hausinger R. P., Legall J., Xavier A. V., and Munck E. , Journal of Biological Chemistry, 1980, Volume 255, Number 6, p.2493-2498, (1980) AbstractWebsite
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Evidence for the formation of a cobalt-iron-sulfur (CoFe3S4) cluster in Desulfovibrio gigas ferredoxin II, Moura, Isabel, Moura Jose J. G., Munck Eckard, Papaefthymiou Vasilios, and Legall Jean , Journal of the American Chemical Society, 1986/01/01, Volume 108, Number 2, p.349-351, (1986) AbstractWebsite
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Influence of storage solution on enamel demineralization submitted to pH cycling, Moura, J. S., Rodrigues L. K., Del Bel Cury A. A., Lima E. M., and Garcia R. M. , J Appl Oral Sci, Sep, Volume 12, Number 3, p.205-8, (2004) AbstractWebsite

Extracted human teeth are frequently used for research or educational purposes. Therefore, it is necessary to store them in disinfectant solutions that do not alter dental structures. Thus, this study evaluated the influence of storage solution on enamel demineralization. For that purpose, sixty samples were divided into the following groups: enamel stored in formaldehyde (F1), stored in thymol (T1), stored in formaldehyde and submitted to pH cycling (F2), stored in thymol and submitted to pH cycling (T2). All samples were evaluated by cross-sectional microhardness analysis and had their percentage of mineral volume versus micrometer (integrated area) determined. Differences between groups were found up to 30-microm depth from the enamel surface (p < 0.05), where samples from group T2 were more demineralized. It was concluded that the storage solution influenced the reaction of a dental substrate to a cariogenic challenge, suggesting that formaldehyde may increase enamel resistance to demineralization, when compared to demineralization occurring in enamel stored in thymol solution.

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