MOLYBDOENZYMES - MECHANISTIC STUDIES AND IDENTIFICATION OF LIGANDS, AT ATOMIC LEVEL 

We have a strong expertise in the structural studies of different metalloenzymes enzymes, in particular Moco-containing enzymes (nitrate reductases, formate dehydrogenases and aldehyde oxidases) from different organisms, and X-ray crystallography methods have helped clarifying the details of enzymatic mechanisms.

• Oliveira, A.R.; Mota, C.; Mourato, C.; Domingos, R.M.; Santos, M.F.A.; Gesto, D.; Guigliarelli, B.; Santos-Silva, T., Romão, MJ; Cardoso Pereira, I. 2020. "Toward the Mechanistic Understanding of Enzymatic CO2 Reduction". ACS Catalysis 10 (6): 3844-3856. 
• The Escherichia coli Periplasmic Aldehyde Oxidoreductase Is an Exceptional Member of the Xanthine Oxidase Family of Molybdoenzymes. Correia, M. A. S., Otrelo-Cardoso, A. R., Schwuchow, V., Sigfridsson Clauss, K. G. V., Haumann, M., Romão, M. J., Leimkühler, S. & Santos-Silva, T., 21 Oct 2016, In : ACS Chemical Biology. 11, 10, p. 2923-2935 13 p.
• Aromatic aldehydes at the active site of aldehyde oxidoreductase from Desulfovibrio gigas: reactivity and molecular details of the enzyme-substrate and enzyme-product interaction. Correia, H. D., Marangon, J., Brondino, C. D., Moura, J. J. G., Romao, M. J., Gonzalez, P. J. & Santos-Silva, T. S., Mar 2015, In : Journal Of Biological Inorganic Chemistry. 20, 2, p. 219-229 11 p.
• Structural and mechanistic insights on nitrate reductases. Coelho, C. M. C. & Romão, M. J., 1 Dec 2015, In : Protein Science. 24, 12, p. 1901-1911 11 p.
• Induced peroxidase activity of haem containing nitrate reductases revealed by protein film electrochemistry. Coelho, C. M. C., Marangon, J., Rodrigues, D., Moura, J. J. G. D., Romão, M. J., Videira, P. D. M. P. D. S. & Correia Dos Santos, M. M., 2013, In : Journal Of Electroanalytical Chemistry. 693, p. 105-113
• Kinetic and Structural Studies of Aldehyde Oxidoreductase from Desulfovibrio gigas Reveal a Dithiolene-Based Chemistry for Enzyme Activation and Inhibition by H2O2. Marangon, J., Correia, H. D., Brondino, C. D., Moura, J. J. G. D., Romão, M. J., Gonzalez, P. J. & Santos-silva, T. S., 2013, In : PLoS ONE. 8, 12, e83234.
• Crystal structure of the zinc-, cobalt-, and iron-containing adenylate kinase from Desulfovibrio gigas: a novel metal-containing adenylate kinase from Gram-negative bacteria. Moura, J. J. G. D., Trincão, J. P. D. S., Bursakov, S. A., Moura, I. M. A. M. G. D. & Romão, M. J., 1 Jan 2011, In : Journal Of Biological Inorganic Chemistry. 16, 1, p. 51-61
• The Crystal Structure of Cupriavidus necator Nitrate Reductase in Oxidized and Partially Reduced States. Moura, J. J. G. D., Trincão, J. P. D. S., Gonzalez, P. J., Moura, I. M. A. M. G. D. & Romão, M. J., 1 Jan 2011, In : Journal of Molecular Biology. 408, 5, p. 932-948
• Molybdenum and tungsten enzymes: a crystallographic and mechanistic overview. Romão, M. J., 1 Jan 2009, In : Dalton Transactions. 21, p. 4053-4068 16 p.

• Crystal structure of the 16 heme cytochrome from Desulfovibrio gigas: A glycosylated protein in a sulphate-reducing bacterium. Moura, I. M. A. M. G. D., Santos-silva, T. S., Pereira, J. M. P. L. & Romão, M. J., 1 Jan 2007, In : Journal of Molecular Biology. 370, 4, p. 659-673