The Macromolecular Crystallography Laboratory & GlycoLab is located at the Chemistry Department of the Faculty of Sciences and Technology, Universidade NOVA de Lisboa.

We integrate the Structural Molecular Biology (SMB) Group of UCIBIO, a research unit from Associate Laboratory REQUIMTE. 

We coordinate the Single Crystal X-ray Structure Determination Service of DQ-FCT-NOVA. 

Main topics of our research

Molybdenum proteins

Nanoparticles and Ionic Liquids for Crystallization

Other metalloproteins

Cellulosome: a cellulose degradation machinery

Drug binding to plasma proteins

Carbohydrate microarrays and ligand discovery

We use X-ray diffraction data provided by diffractometers and/or synchrotron sources to study protein samples using X-ray Crystallography methods:

1. Mechanistic studies and identification of ligands, at atomic level

We have a strong, internationally recognized, expertise in the structural studies of enzymes, like nitrate reductases and aldehyde oxidases from different organisms, and X-ray crystallography methods have helped clarifying the details of several enzymatic mechanisms

2. Drug design and ligand discovery

We use several blood plasma proteins to study the recognition (at atomic level) and/or transport of candidate drugs to treat many diseases. Crystallography data has allowed to "view" the protein-drug interactions, at an atomic level 

3. Glycan-protein and protein-protein interactions

We have used X-ray crystallography, associated to other biophysical methods, to study big molecular assemblies that function like nanomachines. X-ray diffraction, cryo-EM and SAXS data have been crucial to characterize protein modular systems, like cellulosomes. Furthermore, glycans are molecules involved in many recognition and communication processes at the cellular and molecular levels and X-ray data, associated to Glycan Microarrays information, allows to "view" molecular interactions with atomic detail, which is helpful to understand how different molecules behave and recognize partners in the cellular environment.

4. Novel approaches to protein crystallization using nanoparticles and ionic liquids

We use X-ray crystallography methods to assess the effects of innovative agents in crystal growth