May 28, Lecture IYCr2014: João Morais Cabral
Date:
Wednesday, May 28, 2014 (All day) João Morais Cabral
(IBMC, Porto)
A molecular study of a component of the bacterial machinery involved in hyperosmotic adaptation
Abstract
KtrAB is a bacterial K+/Na* transporter that is involved in the first steps of adaptation to hyperosmotic shock. This process involves signaling by ATP/ADP and a change in the conformation of the transporter. I will present the crystal structure of the KtrABATP transporter and will describe biochemical and functional experiments that reveal details of the molecular mechanism of transport activation.
Vieira-Pires RS, Szollosi A, Morais-Cabral JH “The structure of the KtrAB potassium transporter” Nature (2013), 496:323-8
Short biography
Professor João Morais Cabral’s (IBMC, Portugal) education and research experience:
1983/87: ”Licenciatura” in Biochemistry, Universidade do Porto, Porto, Portugal
1987/88: Research assistant at the Analytical section, Dept. of Chemistry, Universidade do Porto, Porto, Portugal
1988/93: Awarded a Ph.D. degree at the University of Edinburgh, Edinburgh,UK
1994/95: Post-doctoral Fellow with Prof. Robert Liddington at Dana-Farber Cancer Institute/Harvard Univ, Boston, USA.
1996/97: Post-doctoral Research Associate with Prof. Robert Liddington at the Biochemistry Dep, Univ of Leicester, UK
1997/2000: Post-doctoral Research Associate with Prof. Roderick MacKinnon at Rockefeller Univ, New York, USA.
2001-2006: Assistant professor at the Dep of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT
2007-2008: Associate Professor at the Dep of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT
2008- : Principal Investigator at the Instituto de Biological Molecular e Celular, Porto, Portugal
6 most recent publications:
• Mari et al “Gating of the MlotiK1 potassium channel involves large rearrangements of the cyclic nucleotide-binding domains”.Proc Natl Acad Sci U S A. (2011), 108:20802-7
• Marques-Carvalho et al “Crystallization and preliminary X-ray crystallographic characterization of a cyclic nucleotide-binding homology domain from the mouse EAG potassium channel” Acta Crystallogr Sect F Struct Biol Cryst Commun. (2012), 68: 337-339
• Harley et al “Changes in Channel Trafficking and Protein Stability Caused by LQT2 Mutations in the PAS Domain of the HERG Channel.” PLoS One (2012), 7(3):e32654.
• Marques-Carvalho et al “Structural, biochemical and functional characterization of the cyclic nucleotide binding homology domain from the mouse EAG1 potassium channel” J Mol Biol. (2012), 423:34-46.
• Adaixo et al “Structural properties of PAS domains from the KCNH potassium channels” PLoS One. (2013);8(3):e59265.
• Vieira-Pires et al “The structure of the KtrAB potassium transporter” Nature (2013), 496:323-8.