Citation:

Portable optoelectronic biosensing platform for identification of mycobacteria from the Mycobacterium tuberculosis complex, Silva, {Leonardo Bione}, Veigas Bruno, c}alo Doria Gon{\c, Costa Pedro, Inácio João, Martins Rodrigo, Fortunato Elvira, and Baptista {Pedro Viana} , Biosensors & Bioelectronics, jan, Volume 26, Number 5, p.2012–2017, (2011)

Abstract:

In this paper we report on the fabrication and performance of a portable and low cost optoelectronic platform integrating a double color tuned light emitting diode as light source, an amorphous/nanocrystalline silicon photodetector with a flat spectral response in the wavelength range from 520. nm to 630. nm and integrated electronic for signal acquisition and conditioning constituted by current to voltage converter, a filter and an amplification stage, followed by an analog to digital converter, with appropriate software for full automation to minimize human error. Incorporation of the double color tuned light emitting diode provides for a simple yet innovative solution to signal acquisition independently from the light intensity and/or solution concentration, while considerably decreasing production costs. Detection based on Au-nanoprobes constitutes the biorecognition step and allowed identification of specific sequences of Mycobacterium tuberculosis complex, namely Mycobacterium bovis and M. tuberculosis in biological samples.

Notes:

This work was partially supported by the Portuguese Science Foundation (FCT-MCTES) for CENIMAT/I3N, CIGMH; Projects PTDC/SAU-BEB/66511/2006, PTDC/EEA-ELC/74236/2006 and PTDC/FIS/74274/2006. SFRH/BD/24665/2005 for L.B.Silva, SFRH/BD/64136/2009 for P. Costa and SFRH/BDE/15544/2005 & STAB Vida, Lda for G. Doria.