Citation:

Polyurea dendrimer for efficient cytosolic siRNA delivery, Restani, {Rita B. }, Conde João, Baptista {Pedro V. }, Cidade {Maria Teresa}, Bragan{\c c}a {Ana M. }, Morgado Jorge, Correia {Ilídio J. }, Aguiar-Ricardo Ana, and Bonifácio {Vasco D. B. } , RSC Advances, Volume 4, Number 97, p.54872–54878, (2014)

Abstract:

The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are 'smart' biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.

Notes:

info:eu-repo/grantAgreement/FCT/COMPETE/132964/PT# info:eu-repo/grantAgreement/FCT/COMPETE/132972/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/116097/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/127178/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/99452/PT# info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F66858%2F2009/PT# PEst-OE/SAU/UI0009/2013 SFRH/BD/62957/2009