Citation:

Immortalization and characterization of a new canine mammary tumour cell line FR37-CMT, Raposo, {L. R. }, Roma-Rodrigues C., Faísca P., Alves M., Henriques J., Carvalheiro {M. C. }, Corvo {M. L. }, Baptista {P. V. }, Pombeiro {A. J. }, and Fernandes {A. R. } , Veterinary and Comparative Oncology, sep, Volume 15, Number 3, p.952–967, (2017)

Abstract:

Here we describe the establishment of a new canine mammary tumour (CMT) cell line, FR37-CMT that does not show dependence on female hormonal signaling to induce tumour xenografts in NOD-SCID mice. FR37-CMT cell line has a stellate or fusiform shape, displays the ability to reorganize the collagen matrix, expresses vimentin, CD44 and shows the loss of E-cadherin which is considered a fundamental event in epithelial to mesenchymal transition (EMT). The up-regulation of ZEB1, the detection of phosphorylated ERK1/2 and the downregulation of DICER1 and miR-200c are also in accordance with the mesenchymal characteristics of FR37-CMT cell line. FR37-CMT shows a higher resistance to cisplatin (IC50>50 µM) and to doxorubicin (IC50>5.3 µM) compared with other CMT cell lines. These results support the use of FR37-CMT as a new CMT model that may assist the understanding of the molecular mechanisms underlying EMT, CMT drug resistance, fostering the development of novel therapies targeting CMT.

Notes:

sem pdf conforme despacho. Fundacao para a Ciencia e a Tecnologia (FCT/MEC) - SFRH/BD/70202/2010 ; UID/Multi/04378/2013 ; UID/DTP/04138/2013.