Citation:

Colorimetric method and kit for the detection of specific nucleic acid sequences using metal nanoparticles functionalized with modified oligonucleotides, Tavares, {Jose Ricardo Ramos Franco}, Baptista {Pedro Miguel Ribeiro Viana}, Doria {Goncalo Maria Reimao Pinto De Franca}, and Flores {Alcino Orfeu De Leao} , nov, (2008)

Abstract:

The present invention relates to a colorimetric method for the detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences, through the aggregation of nanoparticles functionalized with modified oligonucleotides, induced by an increase of the medium's ionic strength. Another aspect of the present invention relates with the development of a kit based on the method of the present invention, allowing for a quick and easy detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences.

Notes:

WO2008135929; C12Q 1/ 68 A I