Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview

Batalha, ÍL, Roque ACA.  2016.  Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview. Phospho-Proteomics. 1355(Methods in Molecular Biology):193–209.


Magnetic nanocomposites are hybrid structures consisting of an iron oxide (Fe3O4 /$\gamma$-Fe2O3 ) superparamagnetic core and a coating shell which presents affi nity for a specifi c target molecule. Within the scope of phosphopeptide enrichment, the magnetic core is usually fi rst functionalized with an intermediate layer of silica or carbon to improve dispersibility and increase specifi c area, and then with an outer layer of a phosphate-affi nity material. Fe3O4 -coating materials include metal oxides, rare earth metal-based compounds, immobilized-metal ions, polymers, and many others. This chapter provides a generic overview of the different materials that can be found in literature and their advantages and drawbacks.



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