Clean synthesis of molecular recognition polymeric materials with chiral sensing capability using supercritical fluid technology. Application as \{HPLC\} stationary phases

Clean synthesis of molecular recognition polymeric materials with chiral sensing capability using supercritical fluid technology. Application as \{HPLC\} stationary phases

Citation:: da Silva, MS, Vão ER, Temtem M, Mafra L, Caldeira J, Aguiar-Ricardo A, Casimiro T. 2010. Clean synthesis of molecular recognition polymeric materials with chiral sensing capability using supercritical fluid technology. Application as \{HPLC\} stationary phases. Biosensors and Bioelectronics. 25:1742-1747., Number 7

Abstract:

Molecularly imprinted polymers (MIPs) of poly(ethylene glycol dimethacrylate) and poly(N-isopropylacrylamide-co-ethylene glycol dimethacrylate) were synthesized for the first time in supercritical carbon dioxide (scCO2), using Boc-l-tryptophan as template. Supercritical fluid technology provides a clean and one-step synthetic route for the preparation of affinity polymeric materials with sensing capability for specific molecules. The polymeric materials were tested as stationary \{HPLC\} phases for the enantiomeric separation of l- and d-tryptophan. \{HPLC\} results prove that the synthesized \{MIPs\} are able to recognize the template molecule towards its enantiomer which opens up potential applications in chromatographic chiral separation.

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