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Mariz, BP, Carvalho S, Batalha IL, Pina AS.  2021.  Artificial enzymes bringing together computational design and directed evolution. Organic & Biomolecular Chemistry. 19(9):1915-1925.
Matos, MJB, Pina AS, Roque ACA.  2020.  Rational design of affinity ligands for bioseparation. Journal of Chromatography A. (460871)
Matos, MJB, Trovão F, Gonçalves J, Rothbauer U, Freire MG, Barbosa AMJB, Pina AS, Roque ACA.  2021.  A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent. Separation and Purification Technology. 265
Maugeri, G, Lychko I, Sobral R, Roque ACA.  2019.  Identification and Antibiotic-Susceptibility Profiling of Infectious Bacterial Agents: A Review of Current and FutureTrends. Biotechnology Journal. 14(1700750) AbstractPDFWebsite

Antimicrobial resistance is one of the most worrying threats to humankind with extremely high healthcare costs associated. The current technologies used in clinical microbiology to identify the bacterial agent and profile antimicrobial susceptibility are time‐consuming and frequently expensive. As a result, physicians prescribe empirical antimicrobial therapies. This scenario is often the cause of therapeutic failures, causing higher mortality rates and healthcare costs, as well as the emergence and spread of antibiotic resistant bacteria. As such, new technologies for rapid identification of the pathogen and antimicrobial susceptibility testing are needed. This review summarizes the current technologies, and the promising emerging and future alternatives for the identification and profiling of antimicrobial resistance bacterial agents, which are expected to revolutionize the field of clinical diagnostics.

Moreira, IP, Esteves C, Palma SICJ, Ramou E, Carvalho ALM, Roque ACA.  2022.  Synergy between silk fibroin and ionic liquids for active gas-sensing materials, jun. Materials Today Bio. 15:100290.: Elsevier AbstractPDFWebsite

Silk fibroin is a biobased material with excellent biocompatibility and mechanical properties, but its use in bioelectronics is hampered by the difficult dissolution and low intrinsic conductivity. Some ionic liquids are known to dissolve fibroin but removed after fibroin processing. However, ionic liquids and fibroin can cooperatively give rise to functional materials, and there are untapped opportunities in this combination. The dissolution of fibroin, followed by gelation, in designer ionic liquids from the imidazolium chloride family with varied alkyl chain lengths (2–10 carbons) is shown here. The alkyl chain length of the anion has a large impact on fibroin secondary structure which adopts unconventional arrangements, yielding robust gels with distinct hierarchical organization. Furthermore, and due to their remarkable air-stability and ionic conductivity, fibroin ionogels are exploited as active electrical gas sensors in an electronic nose revealing the unravelled possibilities of fibroin in soft and flexible electronics.

Moreira, IP, Sato L, Alves C, Palma S, Roque AC.  2021.  Fish gelatin-based films for gas sensing. BIODEVICES 2021 - 14th International Conference on Biomedical Electronics and Devices; Part of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2021. :32–39.: SciTePress Abstract102062.pdf

Electronic noses (e-noses) mimic the complex biological olfactory system, usually including an array of gas sensors to act as the olfactory receptors and a trained computer with signal-processing and pattern recognition tools as the brain. In this work, a new stimuli-responsive material is shown, consisting of self-assembled droplets of liquid crystal and ionic liquid stabilised within a fish gelatin matrix. These materials change their opto/electrical properties upon contact with volatile organic compounds (VOCs). By using an in-house developed e-nose, these new gas-sensing films yield characteristic optical signals for VOCs from different chemical classes. A support vector machine classifier was implemented based on 12 features of the signals. The results show that the films are excellent identifying hydrocarbon VOCs (toluene, heptane and hexane) (95% accuracy) but lower performance was found to other VOCs, resulting in an overall 60.4% accuracy. Even though they are not reusable, these sustainable gas-sensing films are stable throughout time and reproducible, opening several opportunities for future optoelectronic devices and artificial olfaction systems.