Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by ZnCr-AQS LDH
- Citation:
- Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by ZnCr-AQS LDH, Chen, H., Mousty C., Cosnier S., Silveira C., Moura J. J. G., and Almeida M. G. , Electrochemistry Communications, Sep, Volume 9, Number 9, p.2240-2245, (2007)
Abstract:
A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 mu M, a sensitivity of 1.8 A M-1 cm(-2) and a detection limit of 4 nM. The apparent Michaelis-Menten constant (K-M(app)) M was 7.5 mu M. (c) 2007 Elsevier B.V. All rights reserved.
Notes:
Times Cited: 21
