@article {2340, title = {Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by ZnCr-AQS LDH}, journal = {Electrochemistry Communications}, volume = {9}, number = {9}, year = {2007}, note = {

Times Cited: 21

}, month = {Sep}, pages = {2240-2245}, abstract = {

A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 mu M, a sensitivity of 1.8 A M-1 cm(-2) and a detection limit of 4 nM. The apparent Michaelis-Menten constant (K-M(app)) M was 7.5 mu M. (c) 2007 Elsevier B.V. All rights reserved.

}, isbn = {1388-2481}, url = {http://dx.doi.org/10.1016/j.elecom.2007.05.030}, author = {Chen, H. and Mousty, C. and Cosnier, S. and Silveira, C. and Moura, J. J. G. and Almeida, M. G.} }