%0 Journal Article %J Electrochemistry Communications %D 2007 %T Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by ZnCr-AQS LDH %A Chen, H. %A Mousty, C. %A Cosnier, S. %A Silveira, C. %A Moura, J. J. G. %A Almeida, M. G. %M WOS:000249798600017 %P 2240-2245 %U http://dx.doi.org/10.1016/j.elecom.2007.05.030 %V 9 %X

A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 mu M, a sensitivity of 1.8 A M-1 cm(-2) and a detection limit of 4 nM. The apparent Michaelis-Menten constant (K-M(app)) M was 7.5 mu M. (c) 2007 Elsevier B.V. All rights reserved.

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Times Cited: 21

%8 Sep %@ 1388-2481