Coelho, {BJ}, Veigas B, Águas H, Fortunato E, Martins R, Baptista {PV}, Igreja R.
2017.
A digital microfluidics platform for loop-mediated isothermal amplification detection, nov. Sensors. 17, Number 11: MDPI - Multidisciplinary Digital Publishing Institute
AbstractDigital microfluidics (DMF) arises as the next step in the fast-evolving field of operation platforms for molecular diagnostics. Moreover, isothermal schemes, such as loop-mediated isothermal amplification (LAMP), allow for further simplification of amplification protocols. Integrating DMF with LAMP will be at the core of a new generation of detection devices for effective molecular diagnostics at point-of-care (POC), providing simple, fast, and automated nucleic acid amplification with exceptional integration capabilities. Here, we demonstrate for the first time the role of coupling DMF and LAMP, in a dedicated device that allows straightforward mixing of LAMP reagents and target DNA, as well as optimum temperature control (reaction droplets undergo a temperature variation of just 0.3°C, for 65°C at the bottom plate). This device is produced using low-temperature and low-cost production processes, adaptable to disposable and flexible substrates. DMF-LAMP is performed with enhanced sensitivity without compromising reaction efficacy or losing reliability and efficiency, by LAMP-amplifying 0.5 ng/µL of target DNA in just 45 min. Moreover, on-chip LAMP was performed in 1.5 µL, a considerably lower volume than standard bench-top reactions.
Peixoto, D, Figueiredo M, Gawande MB, Corvo MC, Vanhoenacker G, Afonso CAM, Ferreira LM, Branco PS.
2017.
Developments in the Reactivity of 2-Methylimidazolium Salts, JUN 16. JOURNAL OF ORGANIC CHEMISTRY. 82:6232-6241., Number 12
Abstractn/a
Coelho, B, Veigas B, Fortunato E, Martins R, Águas H, Igreja R, Baptista {PV}.
2017.
Digital microfluidics for nucleic acid amplification, jul. Sensors. 17, Number 7: MDPI - Multidisciplinary Digital Publishing Institute
AbstractDigital Microfluidics (DMF) has emerged as a disruptive methodology for the control and manipulation of low volume droplets. In DMF, each droplet acts as a single reactor, which allows for extensive multiparallelization of biological and chemical reactions at a much smaller scale. DMF devices open entirely new and promising pathways for multiplex analysis and reaction occurring in a miniaturized format, thus allowing for healthcare decentralization from major laboratories to point-of-care with accurate, robust and inexpensive molecular diagnostics. Here, we shall focus on DMF platforms specifically designed for nucleic acid amplification, which is key for molecular diagnostics of several diseases and conditions, from pathogen identification to cancer mutations detection. Particular attention will be given to the device architecture, materials and nucleic acid amplification applications in validated settings.