Conde, J, Ambrosone A, Sanz V, Hernandez Y, Marchesano V, Tian F, Child H, Berry {CC }, Ibarra R}{M, Baptista {PV}, Tortiglione C, {de la Fuente} {JM }.
2012.
Design of multifunctional gold nanoparticles for in vitro and in vivo gene silencing, sep. ACS Nano. 6:8316–8324., Number 9: ACS - American Chemical Society
AbstractOver the past decade, the capability of double-stranded RNAs to interfere with gene expression has driven new therapeutic approaches. Since small interfering RNA (siRNAs, 21 base pair double-stranded RNA) was shown to be able to elicit RNA interference (RNAi), efforts were directed toward the development of efficient delivery systems to preserve siRNA bioactivity throughout the delivery route, from the administration site to the target cell. Here we provide evidence of RNAi triggering, specifically silencing c-myc protooncogene, via the synthesis of a library of novel multifunctional gold nanoparticles (AuNPs). The efficiency of the AuNPs is demonstrated using a hierarchical approach including three biological systems of increasing complexity: in vitro cultured human cells, in vivo invertebrate (freshwater polyp, Hydra), and in vivo vertebrate (mouse) models. Our synthetic methodology involved fine-tuning of multiple structural and functional moieties. Selection of the most active functionalities was assisted step-by-step through functional testing that adopted this hierarchical strategy. Merging these chemical and biological approaches led to a safe, nonpathogenic, self-tracking, and universally valid nanocarrier that could be exploited for therapeutic RNAi.
Fournier, G, Hinderer H, Schmid D, Seign R, Baumann M.
2012.
{The new mobility paradigm: Transformation of value chain and business models}, November. Enterprise and Work Innovation Studies. 8:9-40., Number 8
AbstractFour categories of innovations have been identified by Freeman and Perez: incremental innovations, radical innovations, new technological systems (systemic innovations), and technological revolutions or new techno-economic paradigms. New techno-economic paradigms represent changes in technological systems that are so far-reaching in their effects that they have a major influence on the behaviour of the entire economy. Scarcity of oil and external costs like global warming are the key arguments and the main drivers of the change of the current paradigm. They will affect especially the mobility of individuals and the interlinked business models. Novel business models within newly created markets will raise e.g. extended mobility services, activities aiming at the infrastructure, new opportunities in the field of energy transmission and supply and even new strategies of recycling, reusing or reducing the use of resources in order to address global scarcity issues. Especially for the established players of the automotive industry like original equipment manufacturers (OEMs) or 1st and 2nd tier suppliers this implicates opportunities and risks at the same time. But also new players will get the chance to create and enter new markets with new or extended products or services and lead the new value chain. This paper compiles and evaluates current approaches and business models of selected OEMs together with upcoming players. Additionally their positions within the existing value chain are being analyzed and classified. Bringing together the identified drivers of changes with current trends within the automotive industry the authors also show new concepts of extended business models, e.g. the idea of an ecosystem, that have the potential to cause an additional shift of power within the global mobility value chain.
Conde, J, Baptista {PV}, Hernández Y, Sanz V, {de la Fuente} {JM }.
2012.
Modification of plasmid DNA topology by histone-mimetic gold nanoparticles, nov. Nanomedicine. 7:1657–1666., Number 11: Future Medicine Ltd.
AbstractAims: Our aim is to explore whether gold nanoparticles (AuNPs) functionalized with a carboxylated polyethylene glycol (PEG) and protamine (AuNP@PEG@Prot) can modulate - enhance or restrain - DNA condensation, altering DNA conformation and inducing structural changes. Understanding how these nanoconjugates modulate DNA structure, size and shape of DNA condensates, and enable control over the resulting 3D structures is of major biological and therapeutic importance. Materials & methods: Citrate-AuNPs were covered with a dense layer of a hetero-functional octa(ethylene glycol) (SH-EG(8)-COOH). Conjugation of protamine to the AuNP@PEG was achieved by taking advantage of the carboxylated surface previously generated on the surface of the NP and the remaining amino groups from the protamine, using carbodiimide and N-hydroxysulfosuccinimide coupling reactions. Results & conclusion: AuNP@PEG@Prot modulates the structure and topology of DNA, not only for condensation, but also for decondensation, via formation of higher quantities of dimers and multimers, when compared with AuNP@PEG and free protamine.
Sanz, V, Conde J, Ambrosone A, Hernandez Y, Marchesasno V, Estrada {GG }, Ibarra {MR }, Baptista {PV}, Tian F, Tortiglione C, {de la Fuente} {JM }.
2012.
Multifunctional gold nanoparticles for gene silencing, mar. Abstracts Of Papers Of The American Chemical Society. 243: ACS - American Chemical Society
Abstractn/a
Sanz, V, Conde J, Hernández Y, Baptista {PV}, Ibarra {MR }, {de la Fuente} {JM }.
2012.
Effect of PEG biofunctional spacers and TAT peptide on dsRNA loading on gold nanoparticles, jun. Journal Of Nanoparticle Research. 14, Number 6: Kluwer Academic Publishers
AbstractThe surface chemistry of gold nanoparticles (AuNPs) plays a critical role in the self-assembly of thiolated molecules and in retaining the biological function of the conjugated biomolecules. According to the well-established gold-thiol interaction the undefined ionic species on citrate-reduced gold nanoparticle surface can be replaced with a self-assembled monolayer of certain thiolate derivatives and other biomolecules. Understanding the effect of such derivatives in the functionalization of several types of biomolecules, such as PEGs, peptides or nucleic acids, has become a significant challenge. Here, an approach to attach specific biomolecules to the AuNPs (∼14 nm) surface is presented together with a study of their effect in the functionalization with other specific derivatives. The effect of biofunctional spacers such as thiolated poly(ethylene glycol) (PEG) chains and a positive peptide, TAT, in dsRNA loading on AuNPs is reported. Based on the obtained data, we hypothesize that loading of oligonucleotides onto the AuNP surface may be controlled by ionic and weak interactions positioning the entry of the oligo through the PEG layer. We demonstrate that there is a synergistic effect of the TAT peptide and PEG chains with specific functional groups on the enhancement of dsRNA loading onto AuNPs.
Horst, M.
2012.
Ambient Intelligence in Home Care in the Netherlands, 2012. :29., Monte de Caparica: IET
AbstractThe largest age group in the Netherlands is aging and when they are in need of home care, there will not be enough people to take care of them in the current healthcare system. One solution could be found in Ambient Intelligence, for it could aid in maintaining independency and postpone the time that people have to go to a nursing home. Furthermore, it could make the job of the caretakers easier. There are pros and cons to use Ambient Intelligence in this delicate matter that will be discussed in this paper. Furthermore, the implications of employing Ambient Intelligence strategies in home care situations will be considered. The conclusions of a recent study by the Rathenau Institute will be used as the red thread, critically looked at and taken into consideration for the recommendations of how to implement Ambient Intelligence in home care.