Citation:

Alves, PU, Vinhas R, Fernandes AR, Birol SZ, Trabzon L, Bernacka-Wojcik I, Igreja R, Lopes P, Baptista PV, Aguas H, Fortunato E, Martins R.  2018.  Multifunctional microfluidic chip for optical nanoprobe based RNA detection - application to Chronic Myeloid Leukemia, 2018. Sci Rep. 8(1):381.

Abstract:

Many diseases have their treatment options narrowed and end up being fatal if detected during later stages. As a consequence, point-of-care devices have an increasing importance for routine screening applications in the health sector due to their portability, fast analyses and decreased cost. For that purpose, a multifunctional chip was developed and tested using gold nanoprobes to perform RNA optical detection inside a microfluidic chip without the need of molecular amplification steps. As a proof-of-concept, this device was used for the rapid detection of chronic myeloid leukemia, a hemato-oncological disease that would benefit from early stage diagnostics and screening tests. The chip passively mixed target RNA from samples, gold nanoprobes and saline solution to infer a result from their final colorimetric properties. An optical fiber network was used to evaluate its transmitted spectra inside the chip. Trials provided accurate output results within 3 min, yielding signal-to-noise ratios up to 9 dB. When compared to actual state-of-art screening techniques of chronic myeloid leukemia, these results were, at microscale, at least 10 times faster than the reported detection methods for chronic myeloid leukemia. Concerning point-of-care applications, this work paves the way for other new and more complex versions of optical based genosensors.

Notes:

Alves, Pedro UrbanoVinhas, Raquel
Fernandes, Alexandra R
Birol, Semra Zuhal
Trabzon, Levent
Bernacka-Wojcik, Iwona
Igreja, Rui
Lopes, Paulo
Baptista, Pedro Viana
Aguas, Hugo
Fortunato, Elvira
Martins, Rodrigo
eng
Research Support, Non-U.S. Gov't
England
Sci Rep. 2018 Jan 10;8(1):381. doi: 10.1038/s41598-017-18725-9.

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