@article {16126, title = {Multifunctional microfluidic chip for optical nanoprobe based RNA detection - application to Chronic Myeloid Leukemia}, journal = {Sci Rep}, volume = {8}, year = {2018}, note = {

Alves, Pedro UrbanoVinhas, Raquel
Fernandes, Alexandra R
Birol, Semra Zuhal
Trabzon, Levent
Bernacka-Wojcik, Iwona
Igreja, Rui
Lopes, Paulo
Baptista, Pedro Viana
Aguas, Hugo
Fortunato, Elvira
Martins, Rodrigo
eng
Research Support, Non-U.S. Gov{\textquoteright}t
England
Sci Rep. 2018 Jan 10;8(1):381. doi: 10.1038/s41598-017-18725-9.

}, month = {2018}, pages = {381}, abstract = {

Many diseases have their treatment options narrowed and end up being fatal if detected during later stages. As a consequence, point-of-care devices have an increasing importance for routine screening applications in the health sector due to their portability, fast analyses and decreased cost. For that purpose, a multifunctional chip was developed and tested using gold nanoprobes to perform RNA optical detection inside a microfluidic chip without the need of molecular amplification steps. As a proof-of-concept, this device was used for the rapid detection of chronic myeloid leukemia, a hemato-oncological disease that would benefit from early stage diagnostics and screening tests. The chip passively mixed target RNA from samples, gold nanoprobes and saline solution to infer a result from their final colorimetric properties. An optical fiber network was used to evaluate its transmitted spectra inside the chip. Trials provided accurate output results within 3 min, yielding signal-to-noise ratios up to 9 dB. When compared to actual state-of-art screening techniques of chronic myeloid leukemia, these results were, at microscale, at least 10 times faster than the reported detection methods for chronic myeloid leukemia. Concerning point-of-care applications, this work paves the way for other new and more complex versions of optical based genosensors.

}, keywords = {Early Detection of Cancer, Gold, Humans, K562 Cells, Lab-On-A-Chip Devices, Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/*genetics, Metal Nanoparticles, Microfluidic Analytical Techniques/*instrumentation, Optical Fibers, Point-of-Care Systems, RNA/*analysis, Signal-To-Noise Ratio, THP-1 Cells}, isbn = {2045-2322 (Electronic)2045-2322 (Linking) }, url = {https://www.ncbi.nlm.nih.gov/pubmed/29321602}, author = {Alves, P. U. and Vinhas, R. and Fernandes, A. R. and Birol, S. Z. and Trabzon, L. and Bernacka-Wojcik, I. and Igreja, R. and Lopes, P. and P.V. Baptista and Aguas, H. and E. Fortunato and R. Martins} }