Immortalization and characterization of a new canine mammary tumor cell line FR37-CMT
- Citation:
- Raposo, LR, Roma-Rodrigues C, Faísca P, Alves M, Henriques J, Carvalheiro MC, Corvo LM, Baptista PV, Pombeiro AJL, Fernandes AR. 2016. Immortalization and characterization of a new canine mammary tumor cell line FR37-CMT. J. Veterinary and Comparative Oncology.
Abstract:
Here we describe the establishment of a new canine mammary tumour (CMT) cell line, FR37-CMT that does not show dependence on female hormonal signaling to induce tumour xenografts in NOD-SCID mice. FR37-CMT cell line has a stellate or fusiform shape, displays the ability to reorganize the collagen matrix, expresses vimentin, CD44 and shows the loss of E-cadherin which is considered a fundamental event in epithelial to mesenchymal transition (EMT). The up-regulation of ZEB1, the detection of phosphorylated ERK1/2 and the downregulation of DICER1 and miR-200c are also in accordance with the mesenchymal characteristics of FR37-CMT cell line. FR37-CMT shows a higher resistance to cisplatin (IC50>50 µM) and to doxorubicin (IC50>5.3 µM) compared with other CMT cell lines. These results support the use of FR37-CMT as a new CMT model that may assist the understanding of the molecular mechanisms underlying EMT, CMT drug resistance, fostering the development of novel therapies targeting CMT.