@article {11027,
	title = { Immortalization and characterization of a new canine mammary tumor cell line FR37-CMT},
	journal = {J. Veterinary and Comparative Oncology},
	year = {2016},
	abstract = {<p>Here we describe the establishment of a new canine mammary tumour (CMT) cell line, FR37-CMT that does not show dependence on female hormonal signaling to induce tumour xenografts in NOD-SCID mice. FR37-CMT cell line has a stellate or fusiform shape, displays the ability to reorganize the collagen matrix, expresses vimentin, CD44 and shows the loss of E-cadherin which is considered a fundamental event in epithelial to mesenchymal transition (EMT). The up-regulation of ZEB1, the detection of phosphorylated ERK1/2 and the downregulation of DICER1 and miR-200c are also in accordance with the mesenchymal characteristics of FR37-CMT cell line. FR37-CMT shows a higher resistance to cisplatin (IC50\&gt;50 {\textmu}M) and to doxorubicin (IC50\&gt;5.3 {\textmu}M) compared with other CMT cell lines. These results support the use of FR37-CMT as a new CMT model that may assist the understanding of the molecular mechanisms underlying EMT, CMT drug resistance, fostering the development of novel therapies targeting CMT.</p>
},
	url = {http://onlinelibrary.wiley.com/doi/10.1111/vco.12235/abstract},
	author = {Lu{\'\i}s R. Raposo and Catarina Roma-Rodrigues and Pedro Fa{\'\i}sca and Margarida Alves and Henriques, Joaquim and Manuela C. Carvalheiro and Corvo, M. Luisa and Pedro V. Baptista and Armando J. L. Pombeiro and Alexandra R. Fernandes}
}
