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Advances in electrochemically active bacteria: Physiology and ecology. Handbook of Online and Near-real-time Methods in Microbiology. : CRC Press
AbstractThe discovery of microorganisms with the ability of Extracellular Electron Transfer (EET), nearly three decades ago, sparked interest due to their ability to be used in diverse applications that can range from bioremediation to electricity production in Microbial Fuel Cells (MFC). Microbial respiration is based on electron transfer from a donor to an electron acceptor, through a series of stepwise electron transfer events that generate the necessary metabolic energy. Some microorganisms, such as Pseudomonas species, Shewanella putrefaciens or Geothrix fermentans are able to produce electrochemical mediators to increase the EET. The mechanical stability of the biofilm is provided by the biofilm matrix, a hydrated extracellular polymeric matrix that encases the biofilm cells. The biofilm matrix could potentially offer a resistance pathway to EET unless bacteria develop strategies to increase its conductivity. MFC devices currently being used and studied do not generate sufficient power to support widespread and cost-effective applications.
Louro, RO, Salgueiro CA.
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Cytochromes of Shewanella respiratory pathways. Metal Ions in Biology and Medicine - volume 9. (
Alpoim, M.C., Morais, P.V., Santos, MA, Cristovão, AJ, Centeno, JA, Collery, P, Eds.).:236-241., Paris: John Libbey Eurotext
Abstract
Louro, RO, Catarino T, Salgueiro CA, Legall J, Turner DL, Xavier AV.
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Molecular Basis for Energy Transduction: Mechanisms of Cooperativity in Multihaem Cytochromes. Biological Electron Transfer Chains: Genetics, Composition and Mode of Operation NATO ASI Series Volume 512. (
Canters, G.W., Vijgenboom, E., Eds.).:209-223.: Springer Netherlands
AbstractEnergy transduction through electron/proton cooperativity is at the heart of the metabolism of every living organism Nonetheless, the search for the structural bases sustaining these phenomena has been hindered by the fact that they are usually associated with complex transmembrane proteins of high molecular weight.
Salgueiro, CA, Dantas JM, Morgado L.
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Principles of Nuclear Magnetic Resonance and Selected Biological Applications. Radiation in Bioanalysis: Spectroscopic Techniques and Theoretical Methods. (
Pereira, Alice S., Tavares, Pedro, Limão-Vieira, Paulo, Eds.).:245–286., Cham: Springer International Publishing
AbstractNuclear Magnetic Resonance (NMR) spectroscopy is extremely powerful to study distinct biological systems ranging from biomolecules to specific metabolites. This chapter presents the basic concepts of the technique and illustrates its potential to study such systems. Similarly, to other spectroscopic techniques, the theoretical background of NMR is sustained by detailed mathematics and physical chemistry concepts, which were kept to the minimum. The intent is to introduce the fundamentals of the technique to science students from different backgrounds. The basic concepts of NMR spectroscopy are briefly presented in the first section, and the following sections describe applications in the biosciences field, using electron transfer proteins as model, particularly cytochromes. The heme groups endow cytochromes with particular features making them excellent examples to illustrate the high versatility of NMR spectroscopy. The main methodologies underlying protein solution structure determination are discussed in the second section. This is followed by a description of the main experiments explored to structurally map protein-protein or protein-ligand interface regions in molecular complexes. Finally, it is shown how NMR spectroscopy can assist in the functional characterization of multiheme cytochromes.