NMR studies of the interaction between inner membrane-associated and periplasmic cytochromes from Geobacter sulfurreducens

NMR studies of the interaction between inner membrane-associated and periplasmic cytochromes from Geobacter sulfurreducens

Citation:: Dantas, JM, Brausemann A, Einsle O, Salgueiro CA. 2017. NMR studies of the interaction between inner membrane-associated and periplasmic cytochromes from Geobacter sulfurreducens. FEBS Letters. 591:1657–1666.

Abstract:

Geobacter sulfurreducens is a dissimilatory metal reducing bacterium with notable properties and significance in biotechnological applications. Biochemical studies suggest that the inner membrane-associated diheme cytochrome MacA and the periplasmic triheme cytochrome PpcA from G. sulfurreducens can exchange electrons. In this work, NMR chemical shift perturbation measurements were used to map the interface region and to measure the binding affinity between PpcA and MacA. The results show that MacA binds to PpcA in a cleft defined by hemes I and IV, favoring the contact between PpcA heme IV and the MacA high potential heme. The dissociation constant values indicate the formation of a low affinity complex between the proteins, which is consistent with the transient interaction observed in electron transfer complexes.This article is protected by copyright. All rights reserved.

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