Role of vitamin B12 in methyl transfer for methane biosynthesis by Methanosarcina barkeri
- Citation:
- Role of vitamin B12 in methyl transfer for methane biosynthesis by Methanosarcina barkeri, Wood, J. M., Moura I., Moura J. J., Santos M. H., Xavier A. V., Legall J., and Scandellari M. , Science, Apr 16, Volume 216, Number 4543, p.303-5, (1982)
Abstract:
When Methanosarcina barkeri is grown on methanol as the sole carbon source, a B12-containing protein is synthesized by this organism. This B12 protein contains bound aquocobalamin, and when this cofactor is reduced and methylated with [14C]methyl iodide, the resultant [14C]methyl B12 protein is extremely active in the biosynthesis of 14C-labeled methane. These findings indicate that a B12-dependent system is operative in the biological formation of methane in addition to other systems that are B12-independent.
Notes:
0036-8075 (Print)0036-8075 (Linking)Journal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.
