%0 Journal Article %J Science %D 1982 %T Role of vitamin B12 in methyl transfer for methane biosynthesis by Methanosarcina barkeri %A Wood, J. M. %A Moura, I. %A Moura, J. J. %A Santos, M. H. %A Xavier, A. V. %A Legall, J. %A Scandellari, M. %K Bacterial Proteins/metabolism %K Euryarchaeota/*metabolism %K Methane/*biosynthesis %K Methanol/metabolism %K Vitamin B 12/*metabolism %M 7063887 %P 303-5 %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7063887 %V 216 %X

When Methanosarcina barkeri is grown on methanol as the sole carbon source, a B12-containing protein is synthesized by this organism. This B12 protein contains bound aquocobalamin, and when this cofactor is reduced and methylated with [14C]methyl iodide, the resultant [14C]methyl B12 protein is extremely active in the biosynthesis of 14C-labeled methane. These findings indicate that a B12-dependent system is operative in the biological formation of methane in addition to other systems that are B12-independent.

%Z

0036-8075 (Print)0036-8075 (Linking)Journal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

%8 Apr 16