Conversion of desulforedoxin into a rubredoxin center

Conversion of desulforedoxin into a rubredoxin center, Yu, L., Kennedy M., Czaja C., Tavares P., Moura J. J., Moura I., and Rusnak F. , Biochem Biophys Res Commun, Feb 24, Volume 231, Number 3, p.679-82, (1997)


Rubredoxin and desulforedoxin both contain an Fe(S-Cys)4 center. However, the spectroscopic properties of the center in desulforedoxin differ from rubredoxin. These differences arise from a distortion of the metal site hypothesized to result from adjacent cysteine residues in the primary sequence of desulforedoxin. Two desulforedoxin mutants were generated in which either a G or P-V were inserted between adjacent cysteines. Both mutants exhibited optical spectra with maxima at 278, 345, 380, 480, and 560 nm while the low temperature X-band EPR spectra indicated highspin Fe3+ ions with large rhombic distortions (E/D = 0.21-0.23). These spectroscopic properties are distinct from wild type desulforedoxin and virtually identical to rubredoxin.


0006-291X (Print)0006-291X (Linking)Journal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

Related External Link