@article {2474,
	title = {Conversion of desulforedoxin into a rubredoxin center},
	journal = {Biochem Biophys Res Commun},
	volume = {231},
	number = {3},
	year = {1997},
	note = {<p>0006-291X (Print)0006-291X (Linking)Journal ArticleResearch Support, Non-U.S. Gov{\textquoteright}tResearch Support, U.S. Gov{\textquoteright}t, P.H.S.</p>
},
	month = {Feb 24},
	pages = {679-82},
	abstract = {<p>Rubredoxin and desulforedoxin both contain an Fe(S-Cys)4 center. However, the spectroscopic properties of the center in desulforedoxin differ from rubredoxin. These differences arise from a distortion of the metal site hypothesized to result from adjacent cysteine residues in the primary sequence of desulforedoxin. Two desulforedoxin mutants were generated in which either a G or P-V were inserted between adjacent cysteines. Both mutants exhibited optical spectra with maxima at 278, 345, 380, 480, and 560 nm while the low temperature X-band EPR spectra indicated highspin Fe3+ ions with large rhombic distortions (E/D = 0.21-0.23). These spectroscopic properties are distinct from wild type desulforedoxin and virtually identical to rubredoxin.</p>
},
	keywords = {Cysteine/chemistry, Desulfovibrio/chemistry, Electron Spin Resonance Spectroscopy, Iron-Sulfur Proteins/*chemistry, Mass Spectrometry, Mutagenesis, Site-Directed, Rubredoxins/*chemistry, Spectrophotometry, Ultraviolet},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=9070870 },
	author = {Yu, L. and Kennedy, M. and Czaja, C. and Tavares, P and Moura, J. J. and Moura, I. and Rusnak, F.}
}
