Cloning, sequencing and overexpression of the Desulfovibrio gigas ferredoxin gene in E. coli
- Citation:
- Cloning, sequencing and overexpression of the Desulfovibrio gigas ferredoxin gene in E. coli, Chen, B., Menon N. K., Dervertarnian L., Moura J. J., and Przybyla A. E. , FEBS Lett, Sep 12, Volume 351, Number 3, p.401-4, (1994)
Abstract:
We have cloned the gene encoding Desulfovibrio gigas ferredoxin using a photodigoxigenin-labelled probe synthesized with the polymerase chain reaction. The DNA sequence of the gene predicts a polypeptide of 58 residues after removal of the initial formyl methionine (polypeptide M(r) = 6,276). The ferredoxin gene was expressed in aerobically grown E. coli behind the lac promoter of pUC18 resulting in a high level of ferredoxin expression which comprises about 10% of the total cell protein. EPR analysis of recombinant ferredoxin revealed the presence of a [3Fe-4S] cluster which is characteristic of native D. gigas ferredoxin II.
Notes:
0014-5793 (Print)0014-5793 (Linking)Journal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.
