@article {2537,
	title = {Cloning, sequencing and overexpression of the Desulfovibrio gigas ferredoxin gene in E. coli},
	journal = {FEBS Lett},
	volume = {351},
	number = {3},
	year = {1994},
	note = {<p>0014-5793 (Print)0014-5793 (Linking)Journal ArticleResearch Support, Non-U.S. Gov{\textquoteright}tResearch Support, U.S. Gov{\textquoteright}t, P.H.S.</p>
},
	month = {Sep 12},
	pages = {401-4},
	abstract = {<p>We have cloned the gene encoding Desulfovibrio gigas ferredoxin using a photodigoxigenin-labelled probe synthesized with the polymerase chain reaction. The DNA sequence of the gene predicts a polypeptide of 58 residues after removal of the initial formyl methionine (polypeptide M(r) = 6,276). The ferredoxin gene was expressed in aerobically grown E. coli behind the lac promoter of pUC18 resulting in a high level of ferredoxin expression which comprises about 10\% of the total cell protein. EPR analysis of recombinant ferredoxin revealed the presence of a [3Fe-4S] cluster which is characteristic of native D. gigas ferredoxin II.</p>
},
	keywords = {Amino Acid Sequence, Base Sequence, Cloning, Molecular, Desulfovibrio/*genetics, DNA, Bacterial, Escherichia coli/*genetics, Ferredoxins/*genetics, Molecular Sequence Data, Recombinant Proteins/genetics, Spectrum Analysis},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=8082803 },
	author = {Chen, B. and Menon, N. K. and Dervertarnian, L. and Moura, J. J. and Przybyla, A. E.}
}
