Citation:

Characterization of recombinant Desulfovibrio gigas ferredoxin, Rodrigues, P., Graca F., Macedo A. L., Moura I., and Moura J. J. , Biochem Biophys Res Commun, Nov 30, Volume 289, Number 2, p.630-3, (2001)

Abstract:

Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and (1)H NMR data of recombinant and wild-type protein are compared.

Notes:

0006-291X (Print)0006-291X (Linking)Journal ArticleResearch Support, Non-U.S. Gov't

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