@article {2419,
	title = {Characterization of recombinant Desulfovibrio gigas ferredoxin},
	journal = {Biochem Biophys Res Commun},
	volume = {289},
	number = {2},
	year = {2001},
	note = {<p>0006-291X (Print)0006-291X (Linking)Journal ArticleResearch Support, Non-U.S. Gov{\textquoteright}t</p>
},
	month = {Nov 30},
	pages = {630-3},
	abstract = {<p>Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and (1)H NMR data of recombinant and wild-type protein are compared.</p>
},
	keywords = {Cloning, Molecular, Desulfovibrio/*chemistry, Dimerization, Electron Spin Resonance Spectroscopy, Electrophoresis, Polyacrylamide Gel, Escherichia coli/metabolism, Ferredoxins/*chemistry/genetics, Iron/metabolism, Magnetic Resonance Spectroscopy, Oxygen/metabolism, Polymerase Chain Reaction, Recombinant Proteins/*chemistry/metabolism, Spectrophotometry, Sulfur/metabolism, Temperature, Ultraviolet Rays},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=11716522 },
	author = {Rodrigues, P. and Graca, F. and Macedo, A. L. and Moura, I. and Moura, J. J.}
}
