Application of lactate amperometric sol-gel biosensor to sequential injection determination of L-lactate
- Citation:
- Application of lactate amperometric sol-gel biosensor to sequential injection determination of L-lactate, Gomes, S. P., Odlozilikova M., Almeida M. G., Araujo A. N., Couto C. M., and Montenegro M. C. , J Pharm Biomed Anal, Mar 12, Volume 43, Number 4, p.1376-81, (2007)
Abstract:
This work describes the construction and evaluation of lactate sol-gel biosensors to accomplish the determination of lactate in pharmaceutical products. Lactate oxidase was incorporated in a porous sol-gel film placed onto a platinum-based electrode. Acid and basic catalysis were assessed. When coupled to a sequential injection system (SIA) the biosensor, based on (3-aminopropyl)trimethoxysilane, 2-(3,4-epoxycyclohexyl)ethyl-trimethoxysilane, deionised water, polyethylene glycol 6000 and acid catalyst, presented a range of linearity of 5x10(-5) to 5x10(-3)M. The analytical usefulness of the developed biosensor was evaluated through analysis of commercial pharmaceutical products containing lactate with a sampling rate of 40 samples h(-1). The enzyme remained active for at least 30 days, enabling about 700 determinations without sensitivity decrease.
Notes:
Gomes, Sofia PiedadeOdlozilikova, MartinaAlmeida, M GabrielaAraujo, Alberto NCouto, Cristina M C MMontenegro, M Conceicao B S MResearch Support, Non-U.S. Gov'tEnglandJournal of pharmaceutical and biomedical analysisJ Pharm Biomed Anal. 2007 Mar 12;43(4):1376-81. Epub 2007 Jan 4.
