@article {2335,
	title = {Application of lactate amperometric sol-gel biosensor to sequential injection determination of L-lactate},
	journal = {J Pharm Biomed Anal},
	volume = {43},
	number = {4},
	year = {2007},
	note = {<p>Gomes, Sofia PiedadeOdlozilikova, MartinaAlmeida, M GabrielaAraujo, Alberto NCouto, Cristina M C MMontenegro, M Conceicao B S MResearch Support, Non-U.S. Gov{\textquoteright}tEnglandJournal of pharmaceutical and biomedical analysisJ Pharm Biomed Anal. 2007 Mar 12;43(4):1376-81. Epub 2007 Jan 4.</p>
},
	month = {Mar 12},
	pages = {1376-81},
	edition = {2007/01/09},
	abstract = {<p>This work describes the construction and evaluation of lactate sol-gel biosensors to accomplish the determination of lactate in pharmaceutical products. Lactate oxidase was incorporated in a porous sol-gel film placed onto a platinum-based electrode. Acid and basic catalysis were assessed. When coupled to a sequential injection system (SIA) the biosensor, based on (3-aminopropyl)trimethoxysilane, 2-(3,4-epoxycyclohexyl)ethyl-trimethoxysilane, deionised water, polyethylene glycol 6000 and acid catalyst, presented a range of linearity of 5x10(-5) to 5x10(-3)M. The analytical usefulness of the developed biosensor was evaluated through analysis of commercial pharmaceutical products containing lactate with a sampling rate of 40 samples h(-1). The enzyme remained active for at least 30 days, enabling about 700 determinations without sensitivity decrease.</p>
},
	keywords = {*Phase Transition, Biosensing Techniques/instrumentation/*methods, Electrochemistry, Enzymes, Immobilized/*analysis/chemistry, Gels, Kinetics, Lactic Acid/*analysis/chemistry, Pharmaceutical Preparations/analysis/chemistry},
	isbn = {0731-7085 (Print)0731-7085 (Linking)},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=17207600},
	author = {Gomes, S. P. and Odlozilikova, M. and Almeida, M. G. and Araujo, A. N. and Couto, C. M. and Montenegro, M. C.}
}
