Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess,
Nair, R. R., Silveira C. M., Diniz M. S., Almeida M. G., Moura J. J. G., and Rivas M. G.
, J Biol Inorg Chem, Volume 20, p.311–322, (2015)
The 1.4 angstrom resolution structure of Paracoccus pantotrophus pseudoazurin,
Najmudin, Shabir, Pauleta Sofia R., Moura Isabel, and Romao Maria J.
, Acta Crystallographica Section F-Structural Biology and Crystallization Communications, Jun, Volume 66, p.627-635, (2010)
AbstractPseudoazurins are small type 1 copper proteins that are involved in the flow of electrons between various electron donors and acceptors in the bacterial periplasm, mostly under denitrifying conditions. The previously determined structure of Paracoccus pantotrophus pseudoazurin in the oxidized form was improved to a nominal resolution of 1.4 angstrom, with R and R(free) values of 0.188 and 0.206, respectively. This high-resolution structure makes it possible to analyze the interactions between the monomers and the solvent structure in detail. Analysis of the high-resolution structure revealed the structural regions that are responsible for monomer-monomer recognition during dimer formation and for protein-protein interaction and that are important for partner recognition. The pseudoazurin structure was compared with other structures of various type 1 copper proteins and these were grouped into families according to similarities in their secondary structure; this may be useful in the annotation of copper proteins in newly sequenced genomes and in the identification of novel copper proteins.
Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas,
Najmudin, S., Bonifacio C., Duarte A. G., Pauleta S. R., Moura I., Moura J. J., and Romao M. J.
, Acta Crystallogr Sect F Struct Biol Cryst Commun, Jul 1, Volume 65, Number Pt 7, p.730-2, (2009)
AbstractThe orange-coloured protein (ORP) from Desulfovibrio gigas is a 12 kDa protein that contains a novel mixed-metal sulfide cluster of the type [S(2)MoS(2)CuS(2)MoS(2)]. Diffracting crystals of the apo form of ORP have been obtained. Data have been collected for the apo form of ORP to 2.25 A resolution in-house and to beyond 2.0 A resolution at ESRF, Grenoble. The crystals belonged to a trigonal space group, with unit-cell parameters a = 43, b = 43, c = 106 A.
Periplasmic nitrate reductase revisited: a sulfur atom completes the sixth coordination of the catalytic molybdenum,
Najmudin, S., Gonzalez P. J., Trincao J., Coelho C., Mukhopadhyay A., Cerqueira N. M., Romao C. C., Moura I., Moura J. J., Brondino C. D., and Romao M. J.
, J Biol Inorg Chem, Jun, Volume 13, Number 5, p.737-53, (2008)
AbstractNitrate reductase from Desulfovibrio desulfuricans ATCC 27774 (DdNapA) is a monomeric protein of 80 kDa harboring a bis(molybdopterin guanine dinucleotide) active site and a [4Fe-4S] cluster. Previous electron paramagnetic resonance (EPR) studies in both catalytic and inhibiting conditions showed that the molybdenum center has high coordination flexibility when reacted with reducing agents, substrates or inhibitors. As-prepared DdNapA samples, as well as those reacted with substrates and inhibitors, were crystallized and the corresponding structures were solved at resolutions ranging from 1.99 to 2.45 A. The good quality of the diffraction data allowed us to perform a detailed structural study of the active site and, on that basis, the sixth molybdenum ligand, originally proposed to be an OH/OH(2) ligand, was assigned as a sulfur atom after refinement and analysis of the B factors of all the structures. This unexpected result was confirmed by a single-wavelength anomalous diffraction experiment below the iron edge (lambda = 1.77 A) of the as-purified enzyme. Furthermore, for six of the seven datasets, the S-S distance between the sulfur ligand and the Sgamma atom of the molybdenum ligand Cys(A140) was substantially shorter than the van der Waals contact distance and varies between 2.2 and 2.85 A, indicating a partial disulfide bond. Preliminary EPR studies under catalytic conditions showed an EPR signal designated as a turnover signal (g values 1.999, 1.990, 1.982) showing hyperfine structure originating from a nucleus of unknown nature. Spectropotentiometric studies show that reduced methyl viologen, the electron donor used in the catalytic reaction, does not interact directly with the redox cofactors. The turnover signal can be obtained only in the presence of the reaction substrates. With use of the optimized conditions determined by spectropotentiometric titration, the turnover signal was developed with (15)N-labeled nitrate and in D(2)O-exchanged DdNapA samples. These studies indicate that this signal is not associated with a Mo(V)-nitrate adduct and that the hyperfine structure originates from two equivalent solvent-exchangeable protons. The new coordination sphere of molybdenum proposed on the basis of our studies led us to revise the currently accepted reaction mechanism for periplasmic nitrate reductases. Proposals for a new mechanism are discussed taking into account a molybdenum and ligand-based redox chemistry, rather than the currently accepted redox chemistry based solely on the molybdenum atom.
Screening of Potential Stress Biomarkers in Sweat Associated with Sports Training,
Nunes, M. J., Cordas C. M., Moura J. J. G., Noronha J. P., and Branco L. C.
, Sports Medicine - Open, Volume 7, p.8, (2021)
Evaluation of Sweat Sampling Procedures for Human Stress Biomarkers Detection,
Nunes, M. J., Moura J. J. G., Noronha J. P., Branco L. C., Samhan-Arias A., Sousa J. P., Rouco C., and Cordas C.
, Analytica, Volume 3, p.178–194, (2022)
ArsC3 from Desulfovibrio alaskensis G20, a cation and sulfate-independent highly efficient arsenate reductase,
Nunes, C. I., Brás J. L., Najmudin S., Moura J. J. G., Moura I., and Carepo M.
, J Biol Inorg Chem, Volume 19, p.1277-1285, (2014)