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Resonance Raman spectra of rubredoxin: new assignments and vibrational coupling mechanism from iron-54/iron-56 isotope shifts and variable-wavelength excitation, Czernuszewicz, Roman S., Legall Jean, Moura Isabel, and Spiro Thomas G. , Inorganic Chemistry, 1986/02/01, Volume 25, Number 5, p.696-700, (1986) AbstractWebsite
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Purification and characterization of three proteins from a halophilic sulfate-reducing bacterium,<i>Desulfovibrio salexigens</i&gt, Czechowski, M., Fauque G., Galliano N., Dimon B., Moura I., Moura J. J. G., Xavier A. V., Barato B. A. S., Lino A. R., and Legall J. , Journal of Industrial Microbiology & Biotechnology, Volume 1, Number 3, p.139-147, (1986) AbstractWebsite
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Expression of Desulfovibrio gigas desulforedoxin in Escherichia coli. Purification and characterization of mixed metal isoforms, Czaja, C., Litwiller R., Tomlinson A. J., Naylor S., Tavares P., Legall J., Moura J. J., Moura I., and Rusnak F. , J Biol Chem, Sep 1, Volume 270, Number 35, p.20273-7, (1995) AbstractWebsite

The dsr gene from Desulfovibrio gigas encoding the nonheme iron protein desulforedoxin was cloned using the polymerase chain reaction, expressed in Escherichia coli, and purified to homogeneity. The physical and spectroscopic properties of the recombinant protein resemble those observed for the native protein isolated from D. gigas. These include an alpha 2 tertiary structure, the presence of bound iron, and absorbance maxima at 370 and 506 nm in the UV/visible spectrum due to ligand-to-iron charge transfer bands. Low temperature electron paramagnetic resonance studies confirm the presence of a high-spin ferric ion with g values of 7.7, 5.7, 4.1, and 1.8. Interestingly, E. coli produced two forms of desulforedoxin containing iron. One form was identified as a dimer with the metal-binding sites of both subunits occupied by iron while the second form contained equivalent amounts of iron and zinc and represents a dimer with one subunit occupied by iron and the second with zinc.

Cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA), Cunha, C. A., Macieira S., Dias J. M., Almeida G., Goncalves L. L., Costa C., Lampreia J., Huber R., Moura J. J., Moura I., and Romao M. J. , J Biol Chem, May 9, Volume 278, Number 19, p.17455-65, (2003) AbstractWebsite

The gene encoding cytochrome c nitrite reductase (NrfA) from Desulfovibrio desulfuricans ATCC 27774 was sequenced and the crystal structure of the enzyme was determined to 2.3-A resolution. In comparison with homologous structures, it presents structural differences mainly located at the regions surrounding the putative substrate inlet and product outlet, and includes a well defined second calcium site with octahedral geometry, coordinated to propionates of hemes 3 and 4, and caged by a loop non-existent in the previous structures. The highly negative electrostatic potential in the environment around hemes 3 and 4 suggests that the main role of this calcium ion may not be electrostatic but structural, namely in the stabilization of the conformation of the additional loop that cages it and influences the solvent accessibility of heme 4. The NrfA active site is similar to that of peroxidases with a nearby calcium site at the heme distal side nearly in the same location as occurs in the class II and class III peroxidases. This fact suggests that the calcium ion at the distal side of the active site in the NrfA enzymes may have a similar physiological role to that reported for the peroxidases.

Molybdenum EXAFS of the Desulfovibrio gigas Mo(2Fe-2S) protein--structural similarity to "desulfo" xanthine dehydrogenase, Cramer, S. P., Moura J. J., Xavier A. V., and Legall J. , J Inorg Biochem, Apr, Volume 20, Number 4, p.275-80, (1984) AbstractWebsite

The molybdenum EXAFS of the Mo(2Fe-2S) protein from Desulfovibrio gigas has been examined using fluorescence detection and synchrotron radiation. In the oxidized form the molybdenum environment is found to contain two terminal oxo groups and two long (2.47 A) Mo-S bonds. Evidence was also found for an oxygen or nitrogen donor ligand at 1.90 A. Addition of dithionite to the oxidized enzyme results in loss of a terminal oxo group, perhaps due to protonation. In addition, a 0.1 A contraction in the Mo-S bond lengths is observed. The behavior of both oxidized and dithionite-treated forms is similar to that observed previously with "desulfo" xanthine oxidase.

Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774, Costa, C., Moura J. J., Moura I., Wang Y., and Huynh B. H. , J Biol Chem, Sep 20, Volume 271, Number 38, p.23191-6, (1996) AbstractWebsite

The dissimilatory nitrite reductase from Desulfovibrio desulfuricans ATCC 27774 catalyzes the reduction of nitrite to ammonia. Previous spectroscopic investigation revealed that it is a hexaheme cytochrome containing one high spin ferric heme and five low spin ferric hemes in the oxidized enzyme. The current study uses the high resolution of Mossbauer spectroscopy to obtain redox properties of the six heme groups. Correlating the Mossbauer findings with the EPR data reveals the pairwise spin-spin coupling among four of the heme groups. The other two hemes are found to be magnetically isolated. Reduction with dithionite and reaction with CO further indicate that only the high spin heme is capable of binding small exogenous ligands. These results confirm our previous finding that Desulfovibrio desulfuricans nitrite reductase contains six heme groups and that the high spin ferric heme is the substrate and inhibitor binding site.

Hexaheme nitrite reductase from Desulfovibrio desulfuricans. Mossbauer and EPR characterization of the heme groups, Costa, C., Moura J. J., Moura I., Liu M. Y., Peck, H. D. Jr., Legall J., Wang Y. N., and Huynh B. H. , J Biol Chem, Aug 25, Volume 265, Number 24, p.14382-8, (1990) AbstractWebsite

Mossbauer and EPR spectroscopy were used to characterize the heme prosthetic groups of the nitrite reductase isolated from Desulfovibrio desulfuricans (ATCC 27774), which is a membrane-bound multiheme cytochrome capable of catalyzing the 6-electron reduction of nitrite to ammonia. At pH 7.6, the as-isolated enzyme exhibited a complex EPR spectrum consisting of a low-spin ferric heme signal at g = 2.96, 2.28, and 1.50 plus several broad resonances indicative of spin-spin interactions among the heme groups. EPR redox titration studies revealed yet another low-spin ferric heme signal at g = 3.2 and 2.14 (the third g value was undetected) and the presence of a high-spin ferric heme. Mossbauer measurements demonstrated further that this enzyme contained six distinct heme groups: one high-spin (S = 5/2) and five low-spin (S = 1/2) ferric hemes. Characteristic hyperfine parameters for all six hemes were obtained through a detailed analysis of the Mossbauer spectra. D. desulfuricans nitrite reductase can be reduced by chemical reductants, such as dithionite or reduced methyl viologen, or by hydrogenase under hydrogen atmosphere. Addition of nitrite to the fully reduced enzyme reoxidized all five low-spin hemes to their ferric states. The high-spin heme, however, was found to complex NO, suggesting that the high-spin heme could be the substrate binding site and that NO could be an intermediate present in an enzyme-bound form.

Iron compounds after erythrophagocytosis: chemical characterization and immunomodulatory effects, Costa, L. M., Moura E. M., Moura J. J., and de Sousa M. , Biochem Biophys Res Commun, Jun 9, Volume 247, Number 1, p.159-65, (1998) AbstractWebsite

In humans, the lymphomyeloid system has a fundamental role on iron metabolism promoting its recycling due to a continuous removal of effete red blood cells. Additionally, one of the most intriguing aspects of metalloporphyrins in biology is their effect on the immune system. However, the process of erythrocyte catabolism is still poorly understood and needs further research. In the present study, we attempt to investigate the nature and the possible physiologic role of Fe compounds released after erythrophagocytosis during the removal of red blood cells. Monocyte erythrophagocytosis in vitro experiments were done to characterize chemically the Fe compounds present inside the cells and in the culture supernatants. We tested the probable immunomodulatory functions of erythrophagocytosis products over lymphocyte cultures activated in vitro with T mitogens (alpha-CD3). Data obtained from atomic absorption spectroscopy confirmed the presence of Fe in the culture supernatants of monocyte cultures after erythrophagocytosis. Also, high-spin haem complexes derived from erythrocyte catabolism were detected by electron paramagnetic electronic resonance. Finally, in vitro activated lymphocyte proliferation experiments indicate the co-mitogenic properties of monocyte culture supernatants after red blood cells phagocytosis. Thus, the results of the present work provide evidence that culture monocyte supernatants after in vitro erythrophagocytosis contain Fe (III) high-spin haem complexes and show lymphocyte proliferation co-stimulatory properties.

Regulation of the hexaheme nitrite/nitric oxide reductase of Desulfovibrio desulfuricans, Wolinella succinogenes and Escherichia coli. A mass spectrometric study, Costa, C., Macedo A., Moura I., Moura J. J., Legall J., Berlier Y., Liu M. Y., and Payne W. J. , FEBS Lett, Dec 10, Volume 276, Number 1-2, p.67-70, (1990) AbstractWebsite

Dissimilatory nitrite reduction, carried out by hexaheme proteins, gives ammonia as the final product. Representatives of this enzyme group from 3 bacterial species can also reduce NO to either ammonia or N2O. The redox regulation of the nitrite/nitric oxide activities is discussed in the context of the denitrifying pathway.

Formate dehydrogenase from Desulfovibrio desulfuricans ATCC 27774: Isolation and spectroscopic characterization of the active sites (heme, iron-sulfur centers and molybdenum), Costa, C., Teixeira M., Legall J., Moura J. J. G., and Moura I. , Journal of Biological Inorganic Chemistry, Apr, Volume 2, Number 2, p.198-208, (1997) AbstractWebsite

An air-stable formate dehydrogenase, an enzyme that catalyzes the oxidation of formate to CO2, was purified from a sulfate-reducing organism, Desulfovibrio desulfuricans ATCC 27774. The enzyme has a molecular mass of approximately 150 kDa (three different subunits: 88, 29 and 16 kDa) and contains three types of redox-active centers: four c-type hemes, nonheme iron arranged as two [4Fe-4S](2+/1+) centers and a molybdenum-pterin site. Selenium was also chemically detected. The enzyme specific activity is 78 units per mg of protein. Mo(V) EPR signals were observed in the native, reduced and formate-reacted states. EPR signals related to the presence of multiple low-spin hemes were also observed in the oxidized state. Upon reduction, an examination of the EPR data under appropriate conditions distinguishes two types of iron-sulfur centers, an [Fe-S] center I (g(max)=2.050, g(med)=1.947, g(min)=1.896) and an [Fe-S] center II (g(max)=2.071, g(med)=1.926, g(min)=1.865). Mossbauer spectroscopy confirmed the presence of four hemes in the low-spin state. The presence of two [4Fe-4S](2+/1+) centers was confirmed, one of these displaying very small hyperfine coupling constants in the +1 oxidation state. The midpoint redox potentials of the enzyme metal centers were also estimated.

Modelling metallothionein induction in the liver of Sparus aurata exposed to metal-contaminated sediments, Costa, P. M., Repolho T., Caeiro S., Diniz M. E., Moura I., and Costa M. H. , Ecotoxicology and Environmental Safety, Sep, Volume 71, Number 1, p.117-124, (2008) AbstractWebsite

Metallothionein (MT) in the liver of gilthead seabreams (Sparus aurata L., 1758) exposed to Sado estuary (Portugal) sediments was quantified to assess the MT induction potential as a biomarker of sediment-based contamination by copper (Cu), cadmium (U), lead (Pb) and arsenic (As). Sediments were collected from two control sites and four sites with different levels of contamination. Sediment Cu, Cd, Pb, As, total organic matter (TOM) and fine fraction (FF) levels were determined. Generalized linear models (GLM) allowed integration of sediment parameters with liver Cu, Cd, Pb, As and MT concentrations. Although sediment metal levels were lower than expected, we relate NIT with liver Cd and also with interactions between liver and sediment Cu and between liver Cu and TOM. We suggest integrating biomarkers and environmental parameters using statistical models such as GLM as a more sensitive and reliable technique for sediment risk assessment than traditional isolated biomarker approaches. (C) 2007 Elsevier Inc. All rights reserved.

Electrochemical studies of rubredoxin from Desulfovibrio vulgaris at modified electrodes, Correia dos Santos, M. M., Paes de Sousa P. M., Simões Gonçalves M. L., Ascenso C., Moura I., and Moura J. J. G. , Journal of Electroanalytical Chemistry, Volume 501, Number 1–2, p.173-179, (2001) AbstractWebsite
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Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase, Correia dos Santos, M. M., Sousa P. M., Goncalves M. L., Romao M. J., Moura I., and Moura J. J. , Eur J Biochem, Apr, Volume 271, Number 7, p.1329-38, (2004) AbstractWebsite

This work reports on the direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase (DgAOR), a molybdenum enzyme of the xanthine oxidase family that contains three redox-active cofactors: two [2Fe-2S] centers and a molybdopterin cytosine dinucleotide cofactor. The voltammetric behavior of the enzyme was analyzed at gold and carbon (pyrolytic graphite and glassy carbon) electrodes. Two different strategies were used: one with the molecules confined to the electrode surface and a second with DgAOR in solution. In all of the cases studied, electron transfer took place, although different redox reactions were responsible for the voltammetric signal. From a thorough analysis of the voltammetric responses and the structural properties of the molecular surface of DgAOR, the redox reaction at the carbon electrodes could be assigned to the reduction of the more exposed iron cluster, [2Fe-2S] II, whereas reduction of the molybdopterin cofactor occurs at the gold electrode. Voltammetric results in the presence of aldehydes are also reported and discussed.

Electrochemical studies on c-type cytochromes at microelectrodes, Correia dos Santos, M. M., Paes de Sousa P. M., Simões Gonçalves M. L., Lopes H., Moura I., and Moura J. J. G. , Journal of Electroanalytical Chemistry, Volume 464, Number 1, p.76-84, (1999) AbstractWebsite
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Nitrite biosensing using cytochrome c nitrite reductase: Towards a disposable strip electrode, Correia, C., Rodrigues M., Silveira C. M., Moura J. J. G., Ochoteco E., Jubete E., and Almeida M. G. , Biomedical Engineering Systems and Technologies. Series: Communications in Computer and Information Science, (2011)
Cross-linking between cytochrome c3 and flavodoxin from Desulfovibrio gigas, Correia, C., Monzani E., Moura I., Lampreia J., and Moura J. J. , Biochem Biophys Res Commun, Mar 16, Volume 256, Number 2, p.367-71, (1999) AbstractWebsite

Tetraheme cytochrome c3 (13 kDa) and flavodoxin (16 kDa), are small electron transfer proteins that have been used to mimic, in vitro, part of the electron-transfer chain that operates between substract electron donors and respiratory electron acceptors partners in Desulfovibrio species (Palma, N., Moura, I., LeGall, J., Van Beeumen, J., Wampler, J., Moura, J. J. G. (1994) Biochemistry 33, 6394-6407). The electron transfer between these two proteins is believed to occur through the formation of a specific complex where electrostatic interaction is the main driving force (Stewart, D., LeGall, J., Moura, I., Moura, J.J.G., Peck, H.D., Xavier, A.V., Weiner, P.K. and Wampler, J.E. (1988) Biochemistry 27, 2444-2450, Stewart, D., LeGall, J., Moura, I., Moura, J.J.G., Peck, H.D., Xavier, A.V., Weiner, P., Wampler, J. (1989) Eur. J. Biochem. 185, 695-700). In order to obtain structural information of the pre-complex, a covalent complex between the two proteins was prepared. A water-soluble carbodiimide [EDC (1-ethyl-3(3 dimethylaminopropyl) carbodiimide hydrochloride] was used for the cross linking reaction. The reaction was optimized varying a wide number of experimental parameters such as ionic strength, protein and cross linker concentration, and utilization of different cross linkers and reaction time between the crosslinker and proteins.

Biochemical and spectroscopic characterization of the membrane-bound nitrate reductase from Marinobacter hydrocarbonoclasticus 617, Correia, C., Besson S., Brondino C. D., Gonzalez P. J., Fauque G., Lampreia J., Moura I., and Moura J. J. , J Biol Inorg Chem, Nov, Volume 13, Number 8, p.1321-33, (2008) AbstractWebsite

Membrane-bound nitrate reductase from Marinobacter hydrocarbonoclasticus 617 can be solubilized in either of two ways that will ultimately determine the presence or absence of the small (Iota) subunit. The enzyme complex (NarGHI) is composed of three subunits with molecular masses of 130, 65, and 20 kDa. This enzyme contains approximately 14 Fe, 0.8 Mo, and 1.3 molybdopterin guanine dinucleotides per enzyme molecule. Curiously, one heme b and 0.4 heme c per enzyme molecule have been detected. These hemes were potentiometrically characterized by optical spectroscopy at pH 7.6 and two noninteracting species were identified with respective midpoint potentials at Em=+197 mV (heme c) and -4.5 mV (heme b). Variable-temperature (4-120 K) X-band electron paramagnetic resonance (EPR) studies performed on both as-isolated and dithionite-reduced nitrate reductase showed, respectively, an EPR signal characteristic of a [3Fe-4S]+ cluster and overlapping signals associated with at least three types of [4Fe-4S]+ centers. EPR of the as-isolated enzyme shows two distinct pH-dependent Mo(V) signals with hyperfine coupling to a solvent-exchangeable proton. These signals, called "low-pH" and "high-pH," changed to a pH-independent Mo(V) signal upon nitrate or nitrite addition. Nitrate addition to dithionite-reduced samples at pH 6 and 7.6 yields some of the EPR signals described above and a new rhombic signal that has no hyperfine structure. The relationship between the distinct EPR-active Mo(V) species and their plausible structures is discussed on the basis of the structural information available to date for closely related membrane-bound nitrate reductases.

Aromatic aldehydes at the active site of aldehyde oxidoreductase from Desulfovibrio gigas: reactivity and molecular details of the enzyme-substrate and enzyme-product interaction, Correia, H., Marangon J., Brondino C. D., Moura J. J. G., Romao M. J., Gonzalez P. J., and Santos-Silva T. , J Biol Inorg Chem, Volume 20, p.219-229, (2015)
Simplifying sample handling for protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Cordeiro, Francisco M., Carreira Ricardo J., Rial-Otero Raquel, Rivas Gabriela M., Moura Isabel, and Capelo Jose-Luis , Rapid Communications in Mass Spectrometry, 2007, Volume 21, Number 20, p.3269-3278, (2007) AbstractWebsite

An ultrasonic bath, an ultrasonic probe and a sonoreactor were used to speed up the kinetics of the reactions involved in each step of the sample handling for in-gel protein identification by peptide mass fingerprint, PMF, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The following steps were successfully accelerated using ultrasonic energy: gel washing, protein reduction, and protein alkylation. As a result, a reduction comprising 80% to 90% of the total time involved in the classic approach was achieved. In addition the sample handling was also drastically simplified. The number of peptides identified and the protein sequence coverage obtained for the new procedure were comparable to those obtained with the traditional sample treatment for the following protein standards: glycogen phosphorylase b, BSA, ovalbumin, carbonic anhydrase, trypsin inhibitor and alpha-lactalbumin. Finally, as a proof of the procedure, specific proteins were identified from complex protein mixtures obtained from three different sulphate- reducing bacteria: Desulfovibrio, desulfuricans G20, Desulfuvibrio gigas NCIB 9332, and Desulfuvibrio desulfuricans ATCC 27774. Copyright (c) 2007 John Wiley & Sons, Ltd.

Nitric oxide reductase: direct electrochemistry and electrocatalytic activity, Cordas, C. M., Pereira A. S., Martins C. E., Timoteo C. G., Moura I., Moura J. J., and Tavares P. , Chembiochem, Dec, Volume 7, Number 12, p.1878-81, (2006) AbstractWebsite
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Direct electrochemical reduction of carbon dioxide by a molybdenum-containing formate dehydrogenase, Cordas, C. M., Campaniço M., Baptista R., Maia L., Moura I., and Moura J. J. G. , J Inorg Biochem, Volume 196, p.110694, (2019) Website
Comparative electrochemical study of superoxide reductases, Cordas, C. M., Raleiras P., Auchere F., Moura I., and Moura J. J. , Eur Biophys J, Dec 6, Volume 41, Number 12, p.209-215, (2012)
Direct electrochemical study of the multiple redox centers of hydrogenase from Desulfovibrio gigas, Cordas, C. M., Moura I., and Moura J. J. , Bioelectrochemistry, Nov, Volume 74, Number 1, p.83-9, (2008) AbstractWebsite

Direct electrochemical response was first time observed for the redox centers of Desulfovibrio gigas [NiFe]-Hase, in non-turnover conditions, by cyclic voltammetry, in solution at glassy carbon electrode. The activation of the enzyme was achieved by reduction with H(2) and by electrochemical control and electrocatalytic activity was observed. The inactivation of the [NiFe]-Hase was also attained through potential control. All electrochemical data was obtained in the absence of enzyme inhibitors. The results are discussed in the context of the proposed mechanism currently accepted for activation/inactivation of [NiFe]-Hases.

Discovery and characterization of a novel Dyp-type peroxidase from a marine actinobacterium isolated from Trondheim fjord, Norway, Cordas, C. M., Nguyen G. S., Valério G. V., Jønsson M., Sóllner K., Aune I., Wentzel A., and Moura J. J. G. , J Inorg Biochem, Volume 226, p.111651, (2022)
Electrochemical behavior of bacterial nitric oxide reductase – evidences of low redox potential non-heme FeB give new perspectives on the catalytic mechanism, Cordas, C. M., Duarte A. G.,.Moura J. J. G., and Moura I. , Biochim Biophys Acta, Volume 1827, p.233-238, (2013)