Currently, there is high energy consumption in the buildings sector, in Portugal, which implies
an obstacle to the accomplishment of purposes intrinsic to the assumptions of sustainable development
and construction.
Therefore, efforts should be made to allow the construction of energy efficient buildings,
which is based on the adoption of a set of passive and/or active techniques that strive to reduce the
energy consumption of buildings throughout its life cycle, while ensuring comfort for its occupants.
The main objective of this dissertation is to evaluate passive and active proposals for
improving energy efficiency in residential buildings, based on the calculation of energy savings
associated with air conditioning and the payback period. To this end it was established a case study,
using as a base model a building unit of a multifamily building. The initial assessment of energetic
performance of this case study and its comparison after the implementation of improvement strategies
was done through a dynamic analysis using the EnergyPlus software.
The following solutions were evaluated: application of thermal insulation inside the cavity
wall, application of ETICS, more efficient glazing solutions, changing the building's solar orientation,
fixed shading devices application, definition of a pattern of night ventilation in the summer and use of
more efficient electrical equipment and lighting.
It was found that the use of these systems improves the energy efficiency of buildings, such as
the case study, despite of having different payback periods.

The well-known Liouville, Riemann?Liouville and Caputo derivatives are extended to the complex functions space, in a natural way, and it is established interesting connections between them and the Grünwald?Letnikov derivative. Particularly, starting from a complex formulation of the Grünwald?Letnikov derivative we establishes a bridge with existing integral formulations and obtained regularised integrals for Liouville, Riemann?Liouville, and Caputo derivatives. Moreover, it is shown that we can combine the procedures followed in the computation of Riemann?Liouville and Caputo derivatives with the Grünwald?Letnikov to obtain a new way of computing them. The theory we present here will surely open a new way into the fractional derivatives computation.

The present invention relates to a colorimetric method for the detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences, through the aggregation of nanoparticles functionalized with modified oligonucleotides, induced by an increase of the medium's ionic strength. Another aspect of the present invention relates with the development of a kit based on the method of the present invention, allowing for a quick and easy detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences.

The present invention relates to a colorimetric method for the detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences, through the aggregation of nanoparticles functionalized with modified oligonucleotides, induced by an increase of the medium's ionic strength. Another aspect of the present invention relates with the development of a kit based on the method of the present invention, allowing for a quick and easy detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences.

This study reports the comparison of fluorimetric techniques (fluorescence microscopy and spectrofluorimetry on a 96-well format) for the on-bead screening of combinatorial libraries of affinity ligands for chromatographic separations. Two solid-phase libraries of synthetic ligands based on distinct scaffolds were synthesized by combinatorial chemistry. The libraries comprising ligands representing different hydrophobic/hydrophilic properties and sizes were tested for binding to randomly selected biomolecules (labelled with a fluorophore). Fluorescence microscopy was revealed to be a reliable and reproducible technique for the detection of lead ligands which strongly bound the target biomolecule. Results obtained by fluorescence intensity measurements in a 96-well format were less consistent, mainly due to challenges related with the accurate dispensing of the solid support.

A presente Dissertação trata do tema da Construção Sustentável, dando especial enfoque aos Processos Operativos existentes e ao caminho a percorrer para a elaboração de um Processo Operativo enquadrado especificamente dentro dos princípios da Construção Sustentável. Através de uma revisão bibliográfica, procedeu-se ao resumo da evolução dos métodos, técnicas, materiais e fins da construção, desde o início da Humanidade até à actualidade. Apresentou-se, de seguida, o enquadramento geral da realidade nacional e internacional no que respeita à construção de edifícios sustentáveis, tendo em conta as novas premissas para a construção, os novos métodos e os novos processos associados à Construção Sustentável. Neste âmbito, foram analisadas as soluções legislativas vigentes e as propostas analíticas de diversas entidades que se debruçam sobre este tema da Construção Sustentável, enquanto vertente especializada da problemática mais abrangente intrínseca ao aperfeiçoamento e aplicação do conceito de Desenvolvimento Sustentável. Este estudo, integrando uma observação profunda do ciclo de vida do edifício e das especificidades de cada uma das fases que o integram, permitiu cumprir o já referido objectivo primordial da presente Dissertação, contribuindo para o estudo, análise e elaboração de um modelo de investigação que permita avaliar ou elaborar um Processo Operativo para a Construção Sustentável. Desta forma, a presente Dissertação representa um avanço no desenvolvimento de um Processo Operativo para a Construção Sustentável adaptado à realidade da construção em Portugal.