Several lines of recent evidence support a role for chromatin in splicing regulation. Here, we show that splicing can also contribute to histone modification, which implies bidirectional communication between epigenetic mechanisms and RNA processing. Genome-wide analysis of histone methylation in human cell lines and mouse primary T cells reveals that intron-containing genes are preferentially marked with histone H3 Lys36 trimethylation (H3K36me3) relative to intronless genes. In intron-containing genes, H3K36me3 marking is proportional to transcriptional activity, whereas in intronless genes, H3K36me3 is always detected at much lower levels. Furthermore, splicing inhibition impairs recruitment of H3K36 methyltransferase HYPB (also known as Setd2) and reduces H3K36me3, whereas splicing activation has the opposite effect. Moreover, the increase of H3K36me3 correlates with the length of the first intron, consistent with the view that splicing enhances H3 methylation. We propose that splicing is mechanistically coupled to recruitment of HYPB/Setd2 to elongating RNA polymerase II.

The present work proposes the development of a bio-battery composed by an ultrathin monolithic structure of an electrospun cellulose acetate membrane, over which was deposited metallic thin film electrodes by thermal evaporation on both surfaces. The electrochemical characterization of the bio-batteries was performed under simulated body fluids like sweat and blood plasma [salt solution–0.9% (w/w) NaCl]. Reversible electrochemical reactions were detected through the cellulose acetate structure. Thus, a stable electrochemical behavior was achieved for a bio-battery with silver and aluminum thin films as electrodes. This device exhibits the ability to supply a power density higher than 3 muW cm(-2). Finally, a bio-battery prototype was tested on a sweated skin, demonstrating the potential of applicability of this bio-device as a micropower source.

The two-decade long history of events relating object-oriented programming, the development of persistence and transactional support, and the aggregation of multiple nodes in a single-system image cluster, appears to convey the following conclusion: programmers ideally would develop and deploy applications against a single shared global memory space (heap of objects) of mostly unbounded capacity, with implicit support for persistence and concurrency, transparently backed by a possibly large number of clustered physical machines.

In this paper, we propose a new approach to the design of OODB systems for Java applications: (O3)2 (pronounced ozone squared). It aims at providing to developers a single-system image of virtually unbounded object space/heap with support for object persistence, object querying, transactions and concurrency enforcement, backed by a cluster of multi-core machines with Java VMs that is kept transparent to the user/developer. It is based on an existing persistence framework (ozone-db) and the feasibility and performance of our approach has been validated resorting to the OO7 benchmark.